Recombinant Full Length Human SIGLEC12 Protein, C-Flag-tagged

• Cat.No.: SIGLEC12-444HFL
• Product Overview: Recombinant Full Length Human SIGLEC12 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. This gene encodes a member of the SIGLEC3-like subfamily of SIGLECs. Members of this subfamily are characterized by an extracellular V-set immunoglobulin-like domain followed by two C2-set immunoglobulin-like domains, and the cytoplasmic tyrosine-based motifs ITIM and SLAM-like. The encoded protein, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containing protein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negative regulation of macrophage signaling by functioning as an inhibitory receptor. This gene is located in a cluster with other SIGLEC3-like genes on 19q13.4. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 63 kDa
• AA Sequence: MLLLLLLLPPLLCGRVGAKEQKDYL LTMQKSVTVQEGLCVSVLCSFSYPQ NGWTASDPVHGYWFRAGDHV SRNIPVATNNPARAVQEETRDRFHL LGDPQNKDCTLSIRDTRESDAGTYV FCVERGNMKWNYKYDQLSVN VTASQDLLSRYRLEVPESVTVQEGL CVSVPCSVLYPHYNWTASSPVYGSW FKEGADIPWDIPVATNTPSG KVQEDTHGRFLLLGDPQTNNCSLSI RDARKGDSGKYYFQVERGSRKWNYI YDKLSVHVTALTHMPTFSIP GTLESGHPRNLTCSVPWACEQGTPP TITWMGASVSSLDPTITRSSMLSLI PQPQDHGTSLTCQVTLPGAG VTMTRAVRLNISYPPQNLTMTVFQG DGTASTTLRNGSALSVLEGQSLHLV CAVDSNPPARLSWTWGSLTL SPSQSSNLGVLELPRVHVKDEGEFT CRAQNPLGSQHISLSLSLQNEYTGK MRPISGVTLGAFGGAGATAL VFLYFCIIFVVVRSCRKKSARPAVG VGDTGMEDANAVRGSASQGPLIESP ADDSPPHHAPPALATPSPEE
  GEIQYASLSFHKARPQYPQEQEAIG YEYSEINIPKTRTRPLEQKLISEED LAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transmembrane

Gene Information

• Gene Name: SIGLEC12 sialic acid binding Ig like lectin 12 [ Homo sapiens (human) ]
• Official Symbol: SIGLEC12
• Synonyms: S2V; SLG; SIGLECL1; Siglec-XII
• Gene ID: 89858
• mRNA Refseq: NM_053003.4
• Protein Refseq: NP_443729.1
• MIM: 606094
• UniProt ID: Q96PQ1