Recombinant Full Length Human SLIT2 Protein, C-Flag-tagged

• Cat.No.: SLIT2-1147HFL
• Product Overview: Recombinant Full Length Human SLIT2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the slit family of secreted glycoproteins, which are ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. Members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Proteolytic processing of this protein gives rise to an N-terminal fragment that contains the four leucine-rich repeats and five epidermal growth factor repeats and a C-terminal fragment that contains four epidermal growth factor repeats and the cysteine knot. Both full length and cleaved proteins are secreted extracellularly and can function in axon repulsion as well as other specific processes. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 169.7 kDa
• AA Sequence: MRGVGWQMLSLSLGLVLAILNKVAP QACPAQCSCSGSTVDCHGLALRSVP RNIPRNTERLDLNGNNITRI TKTDFAGLRHLRVLQLMENKISTIE RGAFQDLKELERLRLNRNHLQLFPE LLFLGTAKLYRLDLSENQIQ AIPRKAFRGAVDIKNLQLDYNQISC IEDGAFRALRDLEVLTLNNNNITRL SVASFNHMPKLRTFRLHSNN LYCDCHLAWLSDWLRQRPRVGLYTQ CMGPSHLRGHNVAEVQKREFVCSGH QSFMAPSCSVLHCPAACTCS NNIVDCRGKGLTEIPTNLPETITEI RLEQNTIKVIPPGAFSPYKKLRRID LSNNQISELAPDAFQGLRSL NSLVLYGNKITELPKSLFEGLFSLQ LLLLNANKINCLRVDAFQDLHNLNL LSLYDNKLQTIAKGTFSPLR AIQTMHLAQNPFICDCHLKWLADYL HTNPIETSGARCTSPRRLANKRIGQ IKSKKFRCSAKEQYFIPGTE DYRSKLSGDCFADLACPEKCRCEGT TVDCSNQKLNKIPEHIPQYTAELRL NNNEFTVLEATGIFKKLPQL RKINFSNNKITDIEEGAFEGASGVN EILLTSNRLENVQHKMFKGLESLKT LMLRSNRITCVGNDSFIGLS SVRLLSLYDNQITTVAPGAFDTLHS LSTLNLLANPFNCNCYLAWLGEWLR KKRIVTGNPRCQKPYFLKEI PIQDVAIQDFTCDDGNDDNSCSPLS RCPTECTCLDTVVRCSNKGLKVLPK GIPRDVTELYLDGNQFTLVP KELSNYKHLTLIDLSNNRISTLSNQ SFSNMTQLLTLILSYNRLRCIPPRT FDGLKSLRLLSLHGNDISVV PEGAFNDLSALSHLAIGANPLYCDC NMQWLSDWVKSEYKEPGIARCAGPG EMADKLLLTTPSKKFTCQGP VDVNILAKCNPCLSNPCKNDGTCNS DPVDFYRCTCPYGFKGQDCDVPIHA CISNPCKHGGTCHLKEGEED GFWCICADGFEGENCEVNVDDCEDN DCENNSTCVDGINNYTCLCPPEYTG ELCEVKLDFCAQDLNPCQHD SKCILTPKGFKCDCTPGYVGEHCDI DFDDCQDNKCKNGAHCTDAVNGYTC ICPEGYSGLFCEFSPPMVLP RTSPCDNFDCQNGAQCIVRINEPIC QCLPGYQGEKCEKLVSVNFINKESY LQIPSAKVRPQTNITLQIAT DEDSGILLYKGDKDHIAVELYRGRV RASYDTGSHPASAIYSVETINDGNF HIVELLALDQSLSLSVDGGN PKIITNLSKQSTLNFDSPLYVGGMP GKSNVASLRQAPGQNGTSFHGCIRN LYINSELQDFQKVPMQTGIL PGCEPCHKKVCAHGTCQPSSQAGFT CECQEGWMGPLCDQRTNDPCLGNKC VHGTCLPINAFSYSCKCLEG HGGVLCDEEEDLFNPCQAIKCKHGK CRLSGLGQPYCECSSGYTGDSCDRE ISCRGERIRDYYQKQQGYAA
  CQTTKKVSRLECRGGCAGGQCCGPL RSKRRKYSFECTDGSSFVDEVEKVV KCGCTRCVSTRTRPLEQKLISEEDL AANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Axon guidance

Gene Information

• Gene Name: SLIT2 slit guidance ligand 2 [ Homo sapiens (human) ]
• Official Symbol: SLIT2
• Synonyms: SLIL3; Slit-2
• Gene ID: 9353
• mRNA Refseq: NM_004787.4
• Protein Refseq: NP_004778.1
• MIM: 603746
• UniProt ID: O94813