| Description : |
This gene encodes a member of the slit family of secreted glycoproteins, which are ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. Members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Proteolytic processing of this protein gives rise to an N-terminal fragment that contains the four leucine-rich repeats and five epidermal growth factor repeats and a C-terminal fragment that contains four epidermal growth factor repeats and the cysteine knot. Both full length and cleaved proteins are secreted extracellularly and can function in axon repulsion as well as other specific processes. Alternative splicing results in multiple transcript variants. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
169.7 kDa |
| AA Sequence : |
MRGVGWQMLSLSLGLVLAILNKVAP QACPAQCSCSGSTVDCHGLALRSVP RNIPRNTERLDLNGNNITRITKTDF AGLRHLRVLQLMENKISTIERGAFQ DLKELERLRLNRNHLQLFPELLFLG TAKLYRLDLSENQIQAIPRKAFRGA VDIKNLQLDYNQISCIEDGAFRALR DLEVLTLNNNNITRLSVASFNHMPK LRTFRLHSNNLYCDCHLAWLSDWLR QRPRVGLYTQCMGPSHLRGHNVAEV QKREFVCSGHQSFMAPSCSVLHCPA ACTCSNNIVDCRGKGLTEIPTNLPE TITEIRLEQNTIKVIPPGAFSPYKK LRRIDLSNNQISELAPDAFQGLRSL NSLVLYGNKITELPKSLFEGLFSLQ LLLLNANKINCLRVDAFQDLHNLNL LSLYDNKLQTIAKGTFSPLRAIQTM HLAQNPFICDCHLKWLADYLHTNPI ETSGARCTSPRRLANKRIGQIKSKK FRCSAKEQYFIPGTEDYRSKLSGDC FADLACPEKCRCEGTTVDCSNQKLN KIPEHIPQYTAELRLNNNEFTVLEA TGIFKKLPQLRKINFSNNKITDIEE GAFEGASGVNEILLTSNRLENVQHK MFKGLESLKTLMLRSNRITCVGNDS FIGLSSVRLLSLYDNQITTVAPGAF DTLHSLSTLNLLANPFNCNCYLAWL GEWLRKKRIVTGNPRCQKPYFLKEI PIQDVAIQDFTCDDGNDDNSCSPLS RCPTECTCLDTVVRCSNKGLKVLPK GIPRDVTELYLDGNQFTLVPKELSN YKHLTLIDLSNNRISTLSNQSFSNM TQLLTLILSYNRLRCIPPRTFDGLK SLRLLSLHGNDISVVPEGAFNDLSA LSHLAIGANPLYCDCNMQWLSDWVK SEYKEPGIARCAGPGEMADKLLLTT PSKKFTCQGPVDVNILAKCNPCLSN PCKNDGTCNSDPVDFYRCTCPYGFK GQDCDVPIHACISNPCKHGGTCHLK EGEEDGFWCICADGFEGENCEVNVD DCEDNDCENNSTCVDGINNYTCLCP PEYTGELCEVKLDFCAQDLNPCQHD SKCILTPKGFKCDCTPGYVGEHCDI DFDDCQDNKCKNGAHCTDAVNGYTC ICPEGYSGLFCEFSPPMVLPRTSPC DNFDCQNGAQCIVRINEPICQCLPG YQGEKCEKLVSVNFINKESYLQIPS AKVRPQTNITLQIATDEDSGILLYK GDKDHIAVELYRGRVRASYDTGSHP ASAIYSVETINDGNFHIVELLALDQ SLSLSVDGGNPKIITNLSKQSTLNF DSPLYVGGMPGKSNVASLRQAPGQN GTSFHGCIRNLYINSELQDFQKVPM QTGILPGCEPCHKKVCAHGTCQPSS QAGFTCECQEGWMGPLCDQRTNDPC LGNKCVHGTCLPINAFSYSCKCLEG HGGVLCDEEEDLFNPCQAIKCKHGK CRLSGLGQPYCECSSGYTGDSCDRE ISCRGERIRDYYQKQQGYAACQTTK KVSRLECRGGCAGGQCCGPLRSKRR KYSFECTDGSSFVDEVEKVVKCGCT RCVSTRTRPLEQKLISEEDLAANDI LDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
