Description : |
Enables SMAD binding activity; identical protein binding activity; and ubiquitin-protein transferase activity. Involved in negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of trophoblast cell migration; and ubiquitin-dependent SMAD protein catabolic process. Located in nuclear speck. Part of ubiquitin ligase complex. |
Source : |
Mammalian cells |
Species : |
Human |
Tag : |
Flag |
Form : |
25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
Molecular Mass : |
86 kDa |
AA Sequence : |
MSNPGGRRNGPVKLRLTVLCAKNLV KKDFFRLPDPFAKVVVDGSGQCHST DTVKNTLDPKWNQHYDLYIG KSDSVTISVWNHKKIHKKQGAGFLG CVRLLSNAINRLKDTGYQRLDLCKL GPNDNDTVRGQIVVSLQSRD RIGTGGQVVDCSRLFDNDLPDGWEE RRTASGRIQYLNHITRTTQWERPTR PASEYSSPGRPLSCFVDENT PISGTNGATCGQSSDPRLAERRVRS QRHRNYMSRTHLHTPPDLPEGYEQR TTQQGQVYFLHTQTGVSTWH DPRVPRDLSNINCEELGPLPPGWEI RNTATGRVYFVDHNNRTTQFTDPRL SANLHLVLNRQNQLKDQQQQ QVVSLCPDDTECLTVPRYKRDLVQK LKILRQELSQQQPQAGHCRIEVSRE EIFEESYRQVMKMRPKDLWK RLMIKFRGEEGLDYGGVAREWLYLL SHEMLNPYYGLFQYSRDDIYTLQIN PDSAVNPEHLSYFHFVGRIM GMAVFHGHYIDGGFTLPFYKQLLGK SITLDDMELVDPDLHNSLVWILEND ITGVLDHTFCVEHNAYGEII QHELKPNGKSIPVNEENKKEYVRLY VNWRFLRGIEAQFLALQKGFNEVIP QHLLKTFDEKELELIICGLG KIDVNDWKVNTRLKHCTPDSNIVKW FWKAVEFFDEERRARLLQFVTGSSR VPLQGFKALQGAAGPRLFTI HQIDACTNNLPKAHTCFNRIDIPPY ESYEKLYEKLLTAIEETCGFAVETR TRPLEQKLISEEDLAANDILDYKDD DDKV |
Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining. |
Stability : |
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Storage : |
Store at -80 centigrade. |
Concentration : |
>50 ug/mL as determined by microplate BCA method. |
Preparation : |
Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
Protein Families : |
Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors |
Protein Pathways : |
Allograft rejection, Antigen processing and presentation, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Endocytosis, Graft-versus-host disease, TGF-beta signaling pathway, Type I diabetes mellitus, Ubiquitin mediated proteolysis, Viral myocarditis |