Recombinant Full Length Human SPHK2 Protein, C-Flag-tagged

• Cat.No.: SPHK2-1677HFL
• Product Overview: Recombinant Full Length Human SPHK2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 69 kDa
• AA Sequence: MNGHLEAEEQQDQRPDQELTGSWGH GPRSTLVRAKAMAPPPPPLAASTPL LHGEFGSYPARGPRFALTLT SQALHIQRLRPKPEARPRGGLVPLA EVSGCCTLRSRSPSDSAAYFCIYTY PRGRRGARRRATRTFRADGA ATYEENRAEAQRWATALTCLLRGLP LPGDGEITPDLLPRPPRLLLLVNPF GGRGLAWQWCKNHVLPMISE AGLSFNLIQTERQNHARELVQGLSL SEWDGIVTVSGDGLLHEVLNGLLDR PDWEEAVKMPVGILPCGSGN ALAGAVNQHGGFEPALGLDLLLNCS LLLCRGGGHPLDLLSVTLASGSRCF SFLSVAWGFVSDVDIQSERF RALGSARFTLGTVLGLATLHTYRGR LSYLPATVEPASPTPAHSLPRAKSE LTLTPDPAPPMAHSPLHRSV SDLPLPLPQPALASPGSPEPLPILS LNGGGPELAGDWGGAGDAPLSPDPL LSSPPGSPKAALHSPVSEGA PVIPPSSGLPLPTPDARVGASTCGP PDHLLPPLGTPLPPDWVTLEGDFVL MLAISPSHLGADLVAAPHAR FDDGLVHLCWVRSGISRAALLRLFL AMERGSHFSLGCPQLGYAAARAFRL EPLTPRGVLTVDGEQVEYGP
  LQAQMHPGIGTLLTGPPGCPGREPT RTRPLEQKLISEEDLAANDILDYKD DDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Fc gamma R-mediated phagocytosis, Metabolic pathways, Sphingolipid metabolism, VEGF signaling pathway

Gene Information

• Gene Name: SPHK2 sphingosine kinase 2 [ Homo sapiens (human) ]
• Official Symbol: SPHK2
• Synonyms: SK 2; SK-2; SPK 2; SPK-2
• Gene ID: 56848
• mRNA Refseq: NM_020126.5
• Protein Refseq: NP_064511.2
• MIM: 607092
• UniProt ID: Q9NRA0