Recombinant Full Length Human SRF Protein, C-Flag-tagged

• Cat.No.: SRF-597HFL
• Product Overview: Recombinant Full Length Human SRF Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). Two transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 51.4 kDa
• AA Sequence: MLPTQAGAAAALGRGSALGGSLNRT PTGRPGGGGGTRGANGGRVPGNGAG LGPGRLEREAAAAAATTPAP TAGALYSGSEGDSESGEEEELGAER RGLKRSLSEMEIGMVVGGPEASAAA TGGYGPVSGAVSGAKPGKKT RGRVKIKMEFIDNKLRRYTTFSKRK TGIMKKAYELSTLTGTQVLLLVASE TGHVYTFATRKLQPMITSET GKALIQTCLNSPDSPPRSDPTTDQR MSATGFEETDLTYQVSESDSSGETK DTLKPAFTVTNLPGTTSTIQ TAPSTSTTMQVSSGPSFPITNYLAP VSASVSPSAVSSANGTVLKSTGSGP VSSGGLMQLPTSFTLMPGGA VAQQVPVQAIQVHQAPQQASPSRDS STDLTQTSSSGTVTLPATIMTSSVP TTVGGHMMYPSPHAVMYAPT SGLGDGSLTVLNAFSQAPSTMQVSH SQVQEPGGVPQVFLTASSGTVQIPV SAVQLHQMAVIGQQAGSSSN
  LTELQVVNLDTAHSTKSETRTRPLE QKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: MAPK signaling pathway

Gene Information

• Gene Name: SRF serum response factor [ Homo sapiens (human) ]
• Official Symbol: SRF
• Synonyms: MCM1
• Gene ID: 6722
• mRNA Refseq: NM_003131.4
• Protein Refseq: NP_003122.1
• MIM: 600589
• UniProt ID: P11831