Recombinant Full Length Human SRGAP1 Protein, C-Flag-tagged

• Cat.No.: SRGAP1-1784HFL
• Product Overview: Recombinant Full Length Human SRGAP1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a GTPase activator, working with the GTPase CDC42 to negatively regulate neuronal migration. The encoded protein interacts with the transmembrane receptor ROBO1 to inactivate CDC42.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 124.1 kDa
• AA Sequence: MSTPSRFKKDKEIIAEYESQVKEIR AQLVEQQKCLEQQTEMRVQLLQDLQ DFFRKKAEIETEYSRNLEKL AERFMAKTRSTKDHQQYKKDQNLLS PVNCWYLLLNQVRRESKDHATLSDI YLNNVIMRFMQISEDSTRMF KKSKEIAFQLHEDLMKVLNELYTVM KTYHMYHAESISAESKLKEAEKQEE KQIGRSGDPVFHIRLEERHQ RRSSVKKIEKMKEKRQAKYSENKLK SIKARNEYLLTLEATNASVFKYYIH DLSDLIDCCDLGYHASLNRA LRTYLSAEYNLETSRHEGLDIIENA VDNLEPRSDKQRFMEMYPAAFCPPM KFEFQSHMGDEVCQVSAQQP VQAELMLRYQQLQSRLATLKIENEE VKKTTEATLQTIQDMVTIEDYDVSE CFQHSRSTESVKSTVSETYL SKPSIAKRRANQQETEQFYFMKLRE YLEGSNLITKLQAKHDLLQRTLGEG HRAEYMTTRPPNVPPKPQKH RKSRPRSQYNTKLFNGDLETFVKDS GQVIPLIVESCIRFINLYGLQHQGI FRVSGSQVEVNDIKNSFERG ENPLADDQSNHDINSVAGVLKLYFR GLENPLFPKERFNDLISCIRIDNLY ERALHIRKLLLTLPRSVLIV MRYLFAFLNHLSQYSDENMMDPYNL AICFGPTLMPVPEIQDQVSCQAHVN EIIKTIIIHHETIFPDAKEL DGPVYEKCMAGDDYCDSPYSEHGTL EEVDQDAGTEPHTSEDECEPIEAIA KFDYVGRSARELSFKKGASL LLYHRASEDWWEGRHNGIDGLVPHQ YIVVQDMDDTFSDTLSQKADSEASS GPVTEDKSSSKDMNSPTDRH PDGYLARQRKRGEPPPPVRRPGRTS DGHCPLHPPHALSNSSVDLGSPSLA SHPRGLLQNRGLNNDSPERR RRPGHGSLTNISRHDSLKKIDSPPI RRSTSSGQYTGFNDHKPLDPETIAQ DIEETMNTALNELRELERQS TAKHAPDVVLDTLEQVKNSPTPATS TESLSPLHNVALRSSEPQIRRSTSS SSDTMSTFKPMVAPRMGVQL KPPALRPKPAVLPKTNPTIGPAPPP QGPTDKSCTM TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Axon guidance

Gene Information

• Gene Name: SRGAP1 SLIT-ROBO Rho GTPase activating protein 1 [ Homo sapiens (human) ]
• Official Symbol: SRGAP1
• Synonyms: NMTC2; ARHGAP13
• Gene ID: 57522
• mRNA Refseq: NM_020762.4
• Protein Refseq: NP_065813.1
• MIM: 606523
• UniProt ID: Q7Z6B7