Recombinant Full Length Human TP53BP1 Protein, C-Flag-tagged

• Cat.No.: TP53BP1-1891HFL
• Product Overview: Recombinant Full Length Human TP53BP1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a protein that functions in the DNA double-strand break repair pathway choice, promoting non-homologous end joining (NHEJ) pathways, and limiting homologous recombination. This protein plays multiple roles in the DNA damage response, including promoting checkpoint signaling following DNA damage, acting as a scaffold for recruitment of DNA damage response proteins to damaged chromatin, and promoting NHEJ pathways by limiting end resection following a double-strand break. These roles are also important during V(D)J recombination, class switch recombination and at unprotected telomeres. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 213.4 kDa
• AA Sequence: MDPTGSQLDSDFSQQDTPCLIIEDS QPESQVLEDDSGSHFSMLSRHLPNL QTHKENPVLDVVSNPEQTAG EERGDGNSGFNEHLKENKVADPVDS SNLDTCGSISQVIEQLPQPNRTSSV LGMSVESAPAVEEEKGEELE QKEKEKEEDTSGNTTHSLGAEDTAS SQLGFGVLELSQSQDVEENTVPYEV DKEQLQSVTTNSGYTRLSDV DANTAIKHEEQSNEDIPIAEQSSKD IPVTAQPSKDVHVVKEQNPPPARSE DMPFSPKASVAAMEAKEQLS AQELMESGLQIQKSPEPEVLSTQED LFDQSNKTVSSDGCSTPSREEGGCS LASTPATTLHLLQLSGQRSL VQESLSTNSSDLVAPSPDAFRSTPF IVPSSPTEQEGRQDKPMDTSVLSEE GGEPFQKKLQSSEPVELENP PLLPESTVSPQASTPISQSTPVFPP GSLPIPSQPQFSHDIFIPSPSLEEQ SNDGKKDGDMHSSSLTVECS KTSEIEPKNSPEDLGLSLTGDSCKL MLSTSEYSQSPKMESLSSHRIDEDG ENTQIEDTEPMSPVLNSKFV PAENDSILMNPAQDGEVQLSQNDDK TKGDDTDTRDDISILATGCKGREET VAEDVCIDLTCDSGSQAVPS PATRSEALSSVLDQEEAMEIKEHHP EEGSSGSEVEEIPETPCESQGEELK EENMESVPLHLSLTETQSQG LCLQKEMPKKECSEAMEVETSVISI DSPQKLAILDQELEHKEQEAWEEAT SEDSSVVIVDVKEPSPRVDV SCEPLEGVEKCSDSQSWEDIAPEIE PCAENRLDTKEEKSVEYEGDLKSGT AETEPVEQDSSQPSLPLVRA DDPLRLDQELQQPQTQEKTSNSLTE DSKMANAKQLSSDAEAQKLGKPSAH ASQSFCESSSETPFHFTLPK EGDIIPPLTGATPPLIGHLKLEPKR HSTPIGISNYPESTIATSDVMSESM VETHDPILGSGKGDSGAAPD VDDKLCLRMKLVSPETEASEESLQF NLEKPATGERKNGSTAVAESVASPQ KTMSVLSCICEARQENEARS EDPPTTPIRGNLLHFPSSQGEEEKE KLEGDHTIRQSQQPMKPISPVKDPV SPASQKMVIQGPSSPQGEAM VTDVLEDQKEGRSTNQENPSKALIE RPSQNNIGIQTMECSLRVPETVSAA TQTIKNVCEQGTSTVDQNFG KQDATVQTERGSGEKPVSAPGDDTE SLHSQGEEEFDMPQPPHGHVLHRHM RTIREVRTLVTRVITDVYYV DGTEVERKVTEETEEPIVECQECET EVSPSQTGGSSGDLGDISSFSSKAS SLHRTSSGTSLSAMHSSGSS GKGAGPLRGKTSGTEPADFALPSSR GGPGKLSPRKGVSQTGTPVCEEDGD AGLGIRQGGKAPVTPRGRGR RGRPPSRTTGTRETAVPGPLGIEDI SPNLSPDDKSFSRVVPRVPDSTRRT DVGAGALRRSDSPEIPFQAA AGPSDGLDASSPGNSFVGLRVVAKW SSNGYFYSGKITRDVGAGKYKLLFD DGYECDVLGKDILLCDPIPL DTEVTALSEDEYFSAGVVKGHRKES GELYYSIEKEGQRKWYKRMAVILSL EQGNRLREQYGLGPYEAVTP LTKAADISLDNLVEGKRKRRSNVSS PATPTASSSSSTTPTRKITESPRAS MGVLSGKRKLITSEEERSPA KRGRKSATVKPGAVGAGEFVSPCES GDNTGEPSALEEQRGPLPLNKTLFL GYAFLLTMATTSDKLASRSK LPDGPTGSSEEEEEFLEIPPFNKQY TESQLRAGAGYILEDFNEAQCNTAY QCLLIADQHCRTRKYFLCLA SGIPCVSHVWVHDSCHANQLQNYRN YLLPAGYSLEEQRILDWQPRENPFQ NLKVLLVSDQQQNFLELWSE ILMTGGAASVKQHHSSAHNKDIALG VFDVVVTDPSCPASVLKCAEALQLP VVSQEWVIQCLIVGERIGFK QHPKYKHDYVSH TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transcription Factors

Gene Information

• Gene Name: TP53BP1 tumor protein p53 binding protein 1 [ Homo sapiens (human) ]
• Official Symbol: TP53BP1
• Synonyms: p202; 53BP1; TDRD30; p53BP1
• Gene ID: 7158
• mRNA Refseq: NM_005657.4
• Protein Refseq: NP_005648.1
• MIM: 605230
• UniProt ID: Q12888