Recombinant Full Length Human Ubiquitin Protein, Rhodamine 110 Labeled

• Cat.No.: Ubiquitin-07HFL
• Product Overview: This product consists of a full-length, mature ubiquitin polypeptide (amino acids 1-76) recombinantly expressed in E. coli, conjugated on its c-terminus to a quenched Rhodamine 110 dye. Hydrolysis of the conjugate results in fluorescence observable by excitation at 485nm and emission at 535nm.

Specification

• Species: Human
• Source: E.coli
• Protein Length: 1-76aa
• Description: Ubiquitin is a 76 amino acid post-translational modifier expressed throughout all tissues in eukaryotic organisms. The many roles of ubiquitin modification include proteasomal degradation, signal transduction, inflammatory response, and DNA damage repair. Ubiquitin modification occurs through a pyramidal cascade of an E1 activating enzyme, E2 conjugating enzymes ,and an E3 ubiquitin ligases. This enzymatic cascade results in modification of a ε-amine of a lysine residue on a substrate protein. Substrates may either be mono or poly-ubiquitinated by M1, K6, 11, 27, 29, 33, 48 or 63 linkages. Removal of ubiquitin from a substrate protein occurs via deconjugating enzymes, of which there are nearly 100 known enzymes with various specificities.
• Conjugation/Label: Rhodamine 110
• Molecular Mass: 8.9 kDa
• Purity: >99% by LCMS
• Usage: Typical working range: 50-500nM
• Notes: For Research Use Only, Not For Use In Humans.
• Storage: Store at -80 centigrade. Avoid multiple freeze thaw cycles.
• Storage Buffer: 50mM MES, pH 6.0
• Concentration: 300μM, 2.7 mg/mL
• Detection Method: Fluorescence
• Substrate Properties: Protein-Based Substrate
• Excitation/Emission (nm): The Excitation and Emission of this substrate is 485nm and 535nm respectively.