Recombinant Full Length Human UMOD Protein, C-Flag-tagged

• Cat.No.: UMOD-1339HFL
• Product Overview: Recombinant Full Length Human UMOD Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is the most abundant protein in mammalian urine under physiological conditions. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. This protein may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of this protein in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the renal disorders medullary cystic kidney disease-2 (MCKD2), glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI), and familial juvenile hyperuricemic nephropathy (FJHN). Alternative splicing of this gene results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 67.1 kDa
• AA Sequence: MGQPSLTWMLMVVVASWFITTAATD TSEARWCSECHSNATCTEDEAVTTC TCQEGFTGDGLTCVDLDECA IPGAHNCSANSSCVNTPGSFSCVCP EGFRLSPGLGCTDVDECAEPGLSHC HALATCVNVVGSYLCVCPAG YRGDGWHCECSPGSCGPGLDCVPEG DALVCADPCQAHRTLDEYWRSTEYG EGYACDTDLRGWYRFVGQGG ARMAETCVPVLRCNTAAPMWLNGTH PSSDEGIVSRKACAHWSGHCCLWDA SVQVKACAGGYYVYNLTAPP ECHLAYCTDPSSVEGTCEECSIDED CKSNNGRWHCQCKQDFNITDISLLE HRLECGANDMKVSLGKCQLK SLGFDKVFMYLSDSRCSGFNDRDNR DWVSVVTPARDGPCGTVLTRNETHA TYSNTLYLADEIIIRDLNIK INFACSYPLDMKVSLKTALQPMVSA LNIRVGGTGMFTVRMALFQTPSYTQ PYQGSSVTLSTEAFLYVGTM LDGGDLSRFALLMTNCYATPSSNAT DPLKYFIIQDRCPHTRDSTIQVVEN GESSQGRFSVQMFRFAGNYD LVYLHCEVYLCDTMNEKCKPTCSGT RFRSGSVIDQSRVLNLGPITRKGVQ ATVSRAFSSLGLLKVWLPLL
  LSATLTLTFQTRTRPLEQKLISEED LAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: UMOD uromodulin [ Homo sapiens (human) ]
• Official Symbol: UMOD
• Synonyms: THP; FJHN; HNFJ; THGP; HNFJ1; MCKD2; ADTKD1; ADMCKD2
• Gene ID: 7369
• mRNA Refseq: NM_003361.4
• Protein Refseq: NP_003352.2
• MIM: 191845
• UniProt ID: P07911