| Description : |
Notum is an evolutionarily conserved 60 kDa secreted deacylase that regulates Wnt activity. It is tethered to the cell surface by binding to Glypican-like sulfated proteoglycans, and it functions as a Glypican-dependent Wnt inhibitor. Notum hydrolyzes the palmitoyl moiety from Wnt-3a, making the Wnt more hydrophilic and unable to bind to the Frizzled-8 receptor. It also induces the release of cell surface GPI-anchored proteins such as Glypican-3, Cadherin-13, and uPAR. Notum regulates head regeneration in planaria, head formation and neural induction in Xenopus, and long range activity of hedgehog proteins in Drosophila. It is up-regulated in some human hepatocellular carcinomas and colorectal cancers. Within amino acids 88-458, mouse Notum shares 92% and 99% aa sequence identity with human and rat Notum, respectively. |
| Source : |
E. coli |
| Species : |
Mouse |
| Tag : |
His/GST |
| Form : |
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300 |
| Molecular Mass : |
60.8kDa |
| Protein length : |
Glu335~Ser590 |
| Endotoxin : |
<1.0EU per 1µg (determined by the LAL method) |
| Purity : |
> 95% |
| Applications : |
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein |
| Stability : |
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months. |
| Reconstitution : |
Reconstitute in PBS or others. |
