Recombinant Full Length Human VASP Protein, C-Flag-tagged

• Cat.No.: VASP-1409HFL
• Product Overview: Recombinant Full Length Human VASP Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 39.6 kDa
• AA Sequence: MSETVICSSRATVMLYDDGNKRWLP AGTGPQAFSRVQIYHNPTANSFRVV GRKMQPDQQVVINCAIVRGV KYNQATPNFHQWRDARQVWGLNFGS KEDAAQFAAGMASALEALEGGGPPP PPALPTWSVPNGPSPEEVEQ QKRQQPGPSEHIERRVSNAGGPPAP PAGGPPPPPGPPPPPGPPPPPGLPP SGVPAAAHGAGGGPPPAPPL PAAQGPGGGGAGAPGLAAAIAGAKL RKVSKQEEASGGPTAPKAESGRSGG GGLMEEMNAMLARRRKATQV GEKTPKDESANQEEPEARVPAQSES VRRPWEKNSTTLPRMKSSSSVTTSE TQPCTPSSSDYSDLQRVKQE
  LLEEVKKELQKVKEEIIEAFVQELR KRGSPTRTRPLEQKLISEEDLAAND ILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Fc gamma R-mediated phagocytosis, Focal adhesion, Leukocyte transendothelial migration

Gene Information

• Gene Name: VASP vasodilator stimulated phosphoprotein [ Homo sapiens (human) ]
• Official Symbol: VASP
• Synonyms: vasodilator-stimulated phosphoprotein
• Gene ID: 7408
• mRNA Refseq: NM_003370.4
• Protein Refseq: NP_003361.1
• MIM: 601703
• UniProt ID: P50552