Recombinant Human VASP, His-tagged

• Cat.No.: VASP-30994TH
• Product Overview: Recombinant fragment of Human VASP with an N terminal His tag; 363 amino acids with the tag, predicted mwt: 37.5 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 343 amino acids
• Description: Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.
• Conjugation: HIS
• Molecular Weight: 37.500kDa inclusive of tags
• Tissue specificity: Highly expressed in platelets.
• Form: Liquid
• Purity: by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 10% Glycerol, 5mM DTT, 200mM Sodium chloride, 20mM Tris HCl, 0.1mM PMSF, pH 8.0
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MSETVICSSR ATVMLYDDGN KRWLPAGTGP QAFSRVQIYH NPTANSFRVV GRKMQPDQQV VINCAIVRGV KYNQATPNFH QWRDARQVWG LNFGSKEDAA QFAAGMASAL EALEGGGPPP PPALPTWSVP NGPSPEEVEQ QKRQQPGPSE HIERRVSNAG GPPAPPAGGP PPPPGPPPPP GPPPPPGLPP SGVPAAAHGA GGGPPPAPPL PAAQGPGGGG AGAPGLAAAI AGAKLRKVSK QEEASGGPTA PKAESGRSGG GGLMEEMNAM LARRRKATQV GEKTPKDESA NQEEPEARVP AQSESVRRPW EKNSTTLPRM KSSSSVTTSE TQPCTPSSSD YSD
• Sequence Similarities: Belongs to the Ena/VASP family.Contains 1 WH1 domain.

Gene Information

• Gene Name: VASP vasodilator-stimulated phosphoprotein [ Homo sapiens ]
• Official Symbol: VASP
• Synonyms: VASP; vasodilator-stimulated phosphoprotein
• Gene ID: 7408
• mRNA Refseq: NM_003370
• Protein Refseq: NP_003361
• MIM: 601703
• Uniprot ID: P50552
• Chromosome Location: 19q13.2-q13.3
• Pathway: Adaptive Immune System, organism-specific biosystem; Axon guidance, organism-specific biosystem; Cell junction organization, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; Cell-extracellular matrix interactions, organism-specific biosystem
• Function: SH3 domain binding; actin binding; profilin binding; protein binding