Recombinant Full Length Human VPS4A Protein, C-Flag-tagged

• Cat.No.: VPS4A-1245HFL
• Product Overview: Recombinant Full Length Human VPS4A Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. The mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be really an yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 16; the gene for the other resides on chromosome 18.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 48.7 kDa
• AA Sequence: MTTSTLQKAIDLVTKATEEDKAKNY EEALRLYQHAVEYFLHAIKYEAHSD KAKESIRAKCVQYLDRAEKL KDYLRSKEKHGKKPVKENQSEGKGS DSDSEGDNPEKKKLQEQLMGAVVME KPNIRWNDVAGLEGAKEALK EAVILPIKFPHLFTGKRTPWRGILL FGPPGTGKSYLAKAVATEANNSTFF SVSSSDLMSKWLGESEKLVK NLFELARQHKPSIIFIDEVDSLCGS RNENESEAARRIKTEFLVQMQGVGN NNDGTLVLGATNIPWVLDSA IRRRFEKRIYIPLPEEAARAQMFRL HLGSTPHNLTDANIHELARKTEGYS GADISIIVRDSLMQPVRKVQ SATHFKKVCGPSRTNPSMMIDDLLT PCSPGDPGAMEMTWMDVPGDKLLEP VVCMSDMLRSLATTRPTVNA
  DDLLKVKKFSEDFGQESTRTRPLEQ KLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Endocytosis

Gene Information

• Gene Name: VPS4A vacuolar protein sorting 4 homolog A [ Homo sapiens (human) ]
• Official Symbol: VPS4A
• Synonyms: SKD1; SKD2; VPS4; SKD1A; CIMDAG; VPS4-1
• Gene ID: 27183
• mRNA Refseq: NM_013245.3
• Protein Refseq: NP_037377.1
• MIM: 609982
• UniProt ID: Q9UN37