Recombinant Full Length Human WARS1 Protein, C-Flag-tagged

• Cat.No.: WARS1-1851HFL
• Product Overview: Recombinant Full Length Human WARS1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 53 kDa
• AA Sequence: MPNSEPASLLELFNSIATQGELVRS LKAGNASKDEIDSAVKMLVSLKMSY KAAAGEDYKADCPPGNPAPT SNHGPDATEAEEDFVDPWTVQTSSA KGIDYDKLIVRFGSSKIDKELINRI ERATGQRPHHFLRRGIFFSH RDMNQVLDAYENKKPFYLYTGRGPS SEAMHVGHLIPFIFTKWLQDVFNVP LVIQMTDDEKYLWKDLTLDQ AYSYAVENAKDIIACGFDINKTFIF SDLDYMGMSSGFYKNVVKIQKHVTF NQVKGIFGFTDSDCIGKISF PAIQAAPSFSNSFPQIFRDRTDIQC LIPCAIDQDPYFRMTRDVAPRIGYP KPALLHSTFFPALQGAQTKM SASDPNSSIFLTDTAKQIKTKVNKH AFSGGRDTIEEHRQFGGNCDVDVSF MYLTFFLEDDDKLEQIRKDY TSGAMLTGELKKALIEVLQPLIAEH QARRKEVTDEIVKEFMTPRKLSFDF Q TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Aminoacyl-tRNA biosynthesis, Tryptophan metabolism

Gene Information

• Gene Name: WARS1 tryptophanyl-tRNA synthetase 1 [ Homo sapiens (human) ]
• Official Symbol: WARS1
• Synonyms: HMN9; WARS; IFI53; IFP53; GAMMA-2
• Gene ID: 7453
• mRNA Refseq: NM_173701.2
• Protein Refseq: NP_776049.1
• MIM: 191050
• UniProt ID: P23381