Recombinant Full Length Human YARS1 Protein, C-Flag-tagged

• Cat.No.: YARS1-2164HFL
• Product Overview: Recombinant Full Length Human YARS1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 59 kDa
• AA Sequence: MGDAPSPEEKLHLITRNLQEVLGEE KLKEILKERELKIYWGTATTGKPHV AYFVPMSKIADFLKAGCEVT ILFADLHAYLDNMKAPWELLELRVS YYENVIKAMLESIGVPLEKLKFIKG TDYQLSKEYTLDVYRLSSVV TQHDSKKAGAEVVKQVEHPLLSGLL YPGLQALDEEYLKVDAQFGGIDQRK IFTFAEKYLPALGYSKRVHL MNPMVPGLTGSKMSSSEEESKIDLL DRKEDVKKKLKKAFCEPGNVENNGV LSFIKHVLFPLKSEFVILRD EKWGGNKTYTAYVDLEKDFAAEVVH PGDLKNSVEVALNKLLDPIREKFNT PALKKLASAAYPDPSKQKPM AKGPAKNSEPEEVIPSRLDIRVGKI ITVEKHPDADSLYVEKIDVGEAEPR TVVSGLVQFVPKEELQDRLV VVLCNLKPQKMRGVESQGMLLCASI EGINRQVEPLDPPAGSAPGEHVFVK GYEKGQPDEELKPKKKVFEK LQADFKISEECIAQWKQTNFMTKLG SISCKSLKGGNIS TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Aminoacyl-tRNA biosynthesis

Gene Information

• Gene Name: YARS1 tyrosyl-tRNA synthetase 1 [ Homo sapiens (human) ]
• Official Symbol: YARS1
• Synonyms: YRS; YTS; YARS; TYRRS; CMTDIC; IMNEPD2
• Gene ID: 8565
• mRNA Refseq: NM_003680.4
• Protein Refseq: NP_003671.1
• MIM: 603623
• UniProt ID: P54577