Recombinant Full Length Human ZAP70 Protein, C-Flag-tagged

• Cat.No.: ZAP70-885HFL
• Product Overview: Recombinant Full Length Human ZAP70 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 69.7 kDa
• AA Sequence: MPDPAAHLPFFYGSISRAEAEEHLK LAGMADGLFLLRQCLRSLGGYVLSL VHDVRFHHFPIERQLNGTYA IAGGKAHCGPAELCEFYSRDPDGLP CNLRKPCNRPSGLEPQPGVFDCLRD AMVRDYVRQTWKLEGEALEQ AIISQAPQVEKLIATTAHERMPWYH SSLTREEAERKLYSGAQTDGKFLLR PRKEQGTYALSLIYGKTVYH YLISQDKAGKYCIPEGTKFDTLWQL VEYLKLKADGLIYCLKEACPNSSAS NASGAAAPTLPAHPSTLTHP QRRIDTLNSDGYTPEPARITSPDKP RPMPMDTSVYESPYSDPEELKDKKL FLKRDNLLIADIELGCGNFG SVRQGVYRMRKKQIDVAIKVLKQGT EKADTEEMMREAQIMHQLDNPYIVR LIGVCQAEALMLVMEMAGGG PLHKFLVGKREEIPVSNVAELLHQV SMGMKYLEEKNFVHRDLAARNVLLV NRHYAKISDFGLSKALGADD SYYTARSAGKWPLKWYAPECINFRK FSSRSDVWSYGVTMWEALSYGQKPY KKMKGPEVMAFIEQGKRMEC
  PPECPPELYALMSDCWIYKWEDRPD FLTVEQRMRACYYSLASKVEGPPGS TQKAEAACASGPTRTRRLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Natural killer cell mediated cytotoxicity, Primary immunodeficiency, T cell receptor signaling pathway

Gene Information

• Gene Name: ZAP70 zeta chain of T cell receptor associated protein kinase 70 [ Homo sapiens (human) ]
• Official Symbol: ZAP70
• Synonyms: SRK; STD; TZK; STCD; IMD48; ADMIO2; ZAP-70
• Gene ID: 7535
• mRNA Refseq: NM_001079.4
• Protein Refseq: NP_001070.2
• MIM: 176947
• UniProt ID: P43403