Recombinant Full Length Mouse Rad51 Protein, Myc/DDK-tagged

• Cat.No.: Rad51-5350M
• Product Overview: Purified recombinant protein of mouse full-length RAD51 recombinase (Rad51), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR). Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. Also involved in interstrand cross-link repair.
• Molecular Mass: 37 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rad51 RAD51 recombinase [ Mus musculus (house mouse) ]
• Official Symbol: Rad51
• Synonyms: RAD51; RAD51 homolog (S. cerevisiae); DNA repair protein RAD51 homolog 1; RAD51 homolog A; Reca; Rad51a; AV304093
• Gene ID: 19361
• mRNA Refseq: NM_011234
• Protein Refseq: NP_035364
• UniProt ID: Q08297