Human RAD51 Knockdown Cell Lysate

• Cat.No.: RAD51-286HKCL
• Product Overview: WB-validated RAD51 Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 37 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg RAD51 KD HeLa cell lysate
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Homologous recombination, Pancreatic cancer, Pathways in cancer
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: RAD51 RAD51 homolog (S. cerevisiae) [ Homo sapiens (human) ]
• Official Symbol: RAD51
• Synonyms: RAD51; RAD51 homolog (S. cerevisiae); RAD51 (S. cerevisiae) homolog (E coli RecA homolog), RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae), RAD51A, RECA; DNA repair protein RAD51 homolog 1; BRCA1/BRCA2 containing complex; subunit 5; BRCC5; HsRad51; HsT16930; RAD51 homolog A; RecA-like protein; recombination protein A; RecA, E. coli, homolog of; RAD51 homolog (RecA homolog, E. coli); BRCA1/BRCA2-containing complex, subunit 5; RECA; MRMV2; HRAD51; RAD51A
• Gene ID: 5888
• mRNA Refseq: NM_001164269
• Protein Refseq: NP_001157741
• MIM: 179617
• UniProt ID: Q06609