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Recombinant Gaussia princeps Luciferase Protein, Avi-tagged, biotinylated

Cat.No.: Luciferase-153
Product Overview: Recombinant Gaussia princeps Luciferase fused with Avi tag((GLNDIFEAQKIEWHE) tag at C-terminal was expressed in E. coli. Biotinylated.
Description: Luciferase from Gaussia princeps was genetically engineered to be produced in E. coli and to contain the Avitag peptide sequence (GLNDIFEAQKIEWHE, C-terminus). The Avitag™ peptide was biotinylated so one biotin is covalently attached to the lysine within the Avitag peptide.
Source: E. coli
Species: Gaussia princeps
Tag : Avi
Form: 50mM Tris/Cl pH 7.8, 600mM NaCl, 1mM EDTA, <10%BPER II (Pierce)
Molecular Mass: 21,616 Da
Purity: Gluc was purified via affinity
chromatography to greater than 98% purity.
Applications: Gaussia princeps luciferase provides all the research possibilities of the interaction between Streptavidin and biotin with the added applications of bioluminescence.
Storage: The enzyme arrives on dry ice (or, in some cases, ice bricks) and should be immediately stored at -80 centigrade. Protein can be quickly thawed and placed at 4 centigrade. GLuc is stable at this temperature for at least 3 months. Avoid repeated freezethaw cycles.
Shipping: Cold packs required. Store at -20 centigrade or below.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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