Recombinant Hamster Il12b Protein, Met1-Ser327, C-His tagged
Cat.No. : | Il12b-020H |
Product Overview : | Recombinant Hamster Il12b Protein (Met1-Ser327) with C-His tag was expressed in HEK293. |
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Description : | Interleukin 12 p40 subunit (IL-12p40) is a component of the bioactive cytokines IL-12 and IL-23. Existing in either a monomeric or homodimeric form, it is induced in excess of the other subunits of IL-12 and IL23. Most known for providing negative feedback by competing for the IL-12 receptor, it also functions as a chemoattractant for macrophages and is associated with several pathogenic inflammatory responses. |
Source : | HEK293 |
Species : | Hamster |
Tag : | His |
Protein length : | Met1-Ser327 |
Form : | Lyophilized |
Molecular Mass : | Predicted Molecular Mass: 36.5 kDa SDS-PAGE: 45.9 kDa |
Purity : | > 95% as analyzed by SDS-PAGE |
Applications : | ELISA and Western Blot Analysis |
Notes : | For Research Use Only. |
Storage : | 2 years at -20 to -80 centigrade as supplied. 1 month at 4 centigrade after reconstitution with preservative. 1 year at -20 to -80 centigrade after reconstitution. |
Storage Buffer : | Lyophilized from 1×PBS with Trelahose |
Shipping : | Ambient |
Reconstitution : | Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% protein |
Gene Name : | Il12b interleukin 12B [ Cricetulus griseus (Chinese hamster) ] |
Official Symbol : | Il12b |
Synonyms : | Il12b; interleukin 12B; interleukin-12 subunit beta; Interleukin-12 subunit beta |
Gene ID : | 100764758 |
mRNA Refseq : | XM_003502207 |
Protein Refseq : | XP_003502255 |
UniProt ID : | Q8CJE6 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Customer Reviews (3)
Write a reviewGood for cytokine assays.
Effective in Th2 cell differentiation.
High yield in expression.
Q&As (10)
Ask a questionChIP-seq identifies regulatory elements controlling IL12B gene expression.
Reporter assays monitor IL12B promoter activity under varying conditions.
Primary cell stimulation assays gauge IL12B response to pathogens.
siRNA knockdown uncovers IL12B's role in immune cell activation pathways.
Recombinant protein expression enables functional IL12B studies in vitro.
Co-IP validates IL12B's binding to specific signaling partners.
Genetic variant analysis reveals impacts on IL12B protein function.
Flow cytometry profiles IL12B expression across diverse immune cell types.
Cytokine secretion assays measure IL12B's response kinetics to stimuli.
Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.
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