Recombinant Hamster Il12b Protein, Met1-Ser327, C-His tagged

• Cat.No.: Il12b-020H
• Product Overview: Recombinant Hamster Il12b Protein (Met1-Ser327) with C-His tag was expressed in HEK293.

Specification

• Description: Interleukin 12 p40 subunit (IL-12p40) is a component of the bioactive cytokines IL-12 and IL-23. Existing in either a monomeric or homodimeric form, it is induced in excess of the other subunits of IL-12 and IL23. Most known for providing negative feedback by competing for the IL-12 receptor, it also functions as a chemoattractant for macrophages and is associated with several pathogenic inflammatory responses.
• Source: HEK293
• Species: Hamster
• Tag: His
• Protein length: Met1-Ser327
• Form: Lyophilized
• Molecular Mass: Predicted Molecular Mass: 36.5 kDa
  SDS-PAGE: 45.9 kDa
• Purity: > 95% as analyzed by SDS-PAGE
• Applications: ELISA and Western Blot Analysis
• Notes: For Research Use Only.
• Storage: 2 years at -20 to -80 centigrade as supplied.
  1 month at 4 centigrade after reconstitution with preservative.
  1 year at -20 to -80 centigrade after reconstitution.
• Storage Buffer: Lyophilized from 1×PBS with Trelahose
• Shipping: Ambient
• Reconstitution: Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% protein

Gene Information

• Gene Name: Il12b interleukin 12B [ Cricetulus griseus (Chinese hamster) ]
• Official Symbol: Il12b
• Synonyms: Il12b; interleukin 12B; interleukin-12 subunit beta; Interleukin-12 subunit beta
• Gene ID: 100764758
• mRNA Refseq: XM_003502207
• Protein Refseq: XP_003502255
• UniProt ID: Q8CJE6

Reviews

04/08/2020

Good for cytokine assays.

01/06/2020

Effective in Th2 cell differentiation.

01/12/2019

High yield in expression.

Q&As

Could you explain the use of ChIP-seq in studying IL12B transcriptional regulation?

ChIP-seq identifies regulatory elements controlling IL12B gene expression.

Can you elaborate on the use of reporter assays to monitor IL12B promoter activity?

Reporter assays monitor IL12B promoter activity under varying conditions.

What methods evaluate IL12B's response to different pathogenic stimuli in primary cells?

Primary cell stimulation assays gauge IL12B response to pathogens.

How does siRNA knockdown of IL12B elucidate its role in immune cell activation?

siRNA knockdown uncovers IL12B's role in immune cell activation pathways.

How does recombinant protein expression and purification aid in IL12B functional studies?

Recombinant protein expression enables functional IL12B studies in vitro.

How does co-immunoprecipitation validate IL12B's association with signaling partners?

Co-IP validates IL12B's binding to specific signaling partners.

What techniques are employed to analyze the impact of IL12B genetic variants on protein function?

Genetic variant analysis reveals impacts on IL12B protein function.

How does flow cytometry reveal IL12B's expression dynamics in different immune cell subsets?

Flow cytometry profiles IL12B expression across diverse immune cell types.

What experimental approaches determine IL12B's cytokine secretion kinetics upon stimulation?

Cytokine secretion assays measure IL12B's response kinetics to stimuli.

What are the methods to assess IL12B's protein-protein interactions in a cellular context?

Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.