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Recombinant HCoV-OC43 Spike Protein, His-tagged

Cat.No. : Spike-350V
Product Overview : A DNA sequence encoding the Human coronavirus (HCoV-OC43) Spike S1 Protein (AVR40344.1) (Met1-Leu794) was expressed with a polyhistidine tag at the C-terminus.
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Description : The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
Source : HEK293
Species : HCoV-OC43
Tag : His
Form : Lyophilized from sterile PBS, pH 7.4.
Normally 5 % trehalose, 5 % mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Molecular Mass : The recombinant HCoV-OC43 spike S1 consists of 793 amino acids and predicts a molecular mass of 89.02 kDa.
Protein length : Met1-Leu794
Endotoxin : < 1.0 EU per μg protein as determined by the LAL method.
Purity : > 90 % as determined by SDS-PAGE.
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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