| Source : |
HEK293 |
| Species : |
HIV-1 |
| Tag : |
His-Avi |
| Predicted N Terminal : |
Thr 36 |
| Form : |
Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose. |
| Bio-activity : |
Immobilized Human CD4, Fc Tag at 5 ug/mL (100 uL/well) can bind Biotinylated HIV-1 [HIV-1/Clade B/C (CN54)] GP120, His Tag, Avi Tag with a linear range of 0.156-1.25 ug/mL. |
| Molecular Mass : |
The protein has a calculated MW of 56.7 kDa. The protein migrates as 90-116 kDa under reducing condition (SDS-PAGE) due to glycosylation. |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Characteristic : |
Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay. |
| Stability : |
Samples are stable for up to twelve months from date of receipt at -70ºC. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
| Official Symbol : |
GP120 |
| Synonyms : |
GP120, GP120-CN54 |
