Active Recombinant HIV-1 MN TAT Protein

• Cat.No.: HIV1MNTAT-117H

Specification

• Species: HIV
• Source: E.coli
• Tag: Non
• Description: Full length (101 amino acids) Recombinant HIV-1 MN tat produced in the E. coli Expression System. This protein binds to murine monoclonal antibodies of defined epitope specificity and rabbit and human serum polyclonal antibodies (HIV-1 converted serum) in ELISA and Western ELISA.
• Form: Frozen Liquid
• Bio-activity: The biological specificity of this protein was determined by LTR-Beta galactosidase induction in MAGI and MAGI-CCR5 cells. Tat in 1-10ug/ml range induced Beta-galactosidase activity 30-fold over un-treated cells.
• Endotoxin: Less than 0.01 EU/mg of protein as determined by BioWhittaker Kinetic QCL Kit
• Purity: > 95 % by SDS-PAGE and HPLC
• Applications: ELISA;
  Western ELISA;
  Anti-tat Drug Screening;
  Immunization;
  Transcriptional Activation.
• Notes: Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Dilute tat stock solution in saline-phosphate buffer (150mM NaCl, 50mM sodiumphosphate, pH 6.5) immediately before use. Tat readily oxidizes in buffer solutions which may change its LTR- dependent transcriptional activation activity.
  Transcriptional activation assays with tat are performed in 1-10µg/ml range. ELISA and Western ELISA require tat in 10-100ng protein range.
• Stability: At least 6 months at -75 centigrade.
• Storage: Store at -75 centigrade.
• Dilutions: 0.2 KCl, 5mM DTT, 50mM Tris, pH 8.0