Recombinant HIV gag-pol Protein, His-tagged

• Cat.No.: gag-pol-423H
• Product Overview: Recombinant HIV gag-pol (NP_057849.4) (Pro588-Phe1027) was expressed with a polyhistidine tag at the N-terminus.

Specification

• Species: HIV
• Source: E.coli
• Tag: His
• Protein Length: Pro588-Phe1027
• Predicted N Terminal: Met
• Form: Lyophilized from sterile 50 mM Tris, 250 mM Nacl, 1 mM EDTA, 50 % glycerol, pH 7.0. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant p51 (Reverse Transcriptase) of human immunodeficiency virus gag-pol consists 458 amino acids and predicts a molecular mass of 53.6 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Official Symbol: gag-pol
• Synonyms: Gag-Pol;Gag-Pol polyprotein;aspartic peptidase;p66 subunit;integrase;Gag-Pol Transframe peptide;Pol;reverse transcriptase p51 subunit