Recombinant HIV gag-pol Protein, His-tagged


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Recombinant HIV gag-pol Protein, His-tagged

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Cat.No. : gag-pol-423H
Product Overview : Recombinant HIV gag-pol (NP_057849.4) (Pro588-Phe1027) was expressed with a polyhistidine tag at the N-terminus.
Source : E. coli
Species : HIV
Tag : His
Predicted N Terminal : Met
Form : Lyophilized from sterile 50 mM Tris, 250 mM Nacl, 1 mM EDTA, 50 % glycerol, pH 7.0. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Molecular Mass : The recombinant p51 (Reverse Transcriptase) of human immunodeficiency virus gag-pol consists 458 amino acids and predicts a molecular mass of 53.6 kDa.
Protein length : Pro588-Phe1027
Endotoxin : < 1.0 EU per μg protein as determined by the LAL method.
Purity : > 90 % as determined by SDS-PAGE.
Storage : Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Official Symbol : gag-pol
Synonyms : Gag-Pol;Gag-Pol polyprotein;aspartic peptidase;p66 subunit;integrase;Gag-Pol Transframe peptide;Pol;reverse transcriptase p51 subunit

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◆ Recombinant Protein

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