Recombinant HIV1 Gag-Pol Protein, His&Trx tagged

• Cat.No.: gag-pol-001H
• Product Overview: Recombinant HIV1 p24 Protein (133-363) with a TRX and His tag was expressed in E. coli.

Specification

• Species: HIV
• Source: E.coli
• Tag: His&Trx
• Protein Length: 133-363aa
• Description: Human immunodeficiency virus (HIV) is a retrovirusthat can lead to a condition in which the immune systembegins to fail, leading to opportunistic infections. HIV primarily infects vital cells in the humanimmune systemsuch as helper T cells (specifically CD4+ T cells), macrophagesand dendritic cells. HIV infection leads to low levels of CD4+ T cells through three main mechanisms: firstly, direct viral killing of infected cells; secondly, increased rates of apoptosisin infected cells; and thirdly, killing of infected CD4+ T cells by CD8 cytotoxic lymphocytesthat recognize infected cells. HIV was classified as a member of the genus Lentivirus,part of the family of Retroviridae. Lentiviruses have many common morphologies and biological properties. Many species are infected by lentiviruses, which are characteristically responsible for long-duration illnesses with a long incubation period. Lentiviruses are transmitted as single-stranded, positive-sense, enveloped RNA viruses. Upon entry of the target cell, the viral RNA genomeis converted to double-stranded DNA by a virally encoded reverse transcriptasethat is present in the virus particle. This viral DNA is then integrated into the cellular DNA by a virally encoded integraseso that the genome can be transcribed. HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
• Tag: His&Trx
• Endotoxin: Not tested.
• Storage: For long term storage, it is recommended to store at -20 or -80 centigrade in appropriate aliquots. Avoid repeated freeze-thaw cycles.
• Storage Buffer: 1×PBS, pH 7.4.

Gene Information

• Gene Name: gag-pol Gag-Pol [ Human immunodeficiency virus 1 ]
• Official Symbol: gag-pol
• Synonyms: gag-pol; Gag-Pol; Gag-Pol; Gag-Pol polyprotein; aspartic peptidase; p66 subunit; integrase; Gag-Pol Transframe peptide; Pol; reverse transcriptase p51 subunit; Pr160
• Gene ID: 155348
• Protein Refseq: NP_057849.4
• UniProt ID: P04585