Recombinant Human 3-Phosphoinositide Dependent Protein Kinase-1, His-tagged

• Cat.No.: PDPK1-1891H
• Product Overview: Recombinant humanPDPK1 was expressed in insect cells. Its molecular weight is 66900 Dalton.

Specification

• Cat. No.: PDPK1-1891H
• Description: PDPK1 is aserine/threonine protein kinase localized to the plasma membrane. It plays avital role in insulin-induced glycogen synthesis, PKC activation, andactivation of the AGC subfamily of protein kinases. No special measures weretaken to activate this kinase. PDPK1 is a master kinase, which is crucial forthe activation of AKT/PKB and many other AGC kinases including PKC, S6K, SGK.An important role for PDPK1 is in the signalling pathways activated byseveral growth factors and hormones including insulin signalling.
• Source: Insect cells
• Species: Human
• Form: Liquid in 50 mMTris, pH 7.5 + 150 mM NaCl + 0.5 mM EDTA + 0.02% Triton X-100 + 2 mM DTT +50% glycerol.
• Molecular Weight: 66.9 kDa
• Storage: Stable for 6 monthsin working aliquots at -80°C. Avoid repeated freeze-thaw cycles.
• OfficialSymbol: PDPK1

Gene Information

• Gene Name: PDPK1 3-phosphoinositide dependent proteinkinase-1 [ Homo sapiens ]
• Synonyms: PDPK1; 3-phosphoinositidedependent protein kinase-1; 3-phosphoinositide-dependent protein kinase 1;PDK1; PRO0461; MGC20087; MGC35290; PkB kinase; hPDK1; EC 2.7.11.1
• Gene ID: 5170
• mRNA Refseq: NM_002613
• Protein Refseq: NP_002604
• MIM: 605213
• UniProt ID: O15530
• Chromosome Location: 16p13.3
• Pathway: Activation of NMDA receptorupon glutamate binding and postsynaptic events; Activation of PKB; AdaptiveImmune System; Aldosterone-regulated sodium reabsorption; B Cell ReceptorSignaling Pathway; BCR signaling pathway; CD28 co-stimulation; CD28 dependentPI3K/Akt signaling; Class I PI3K signaling events; Costimulation by the CD28family; Focal adhesion; Hemostasis; IGF1 pathway; IRS-mediated Signaling;Insulin Pathway
• Function: 3-phosphoinositide-dependentprotein kinase activity; ATP binding; kinase activity; nucleotide binding;protein binding; protein serine/threonine kinase activity