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Recombinant Human ACAT2 Protein, GST-Tagged

Cat.No. : ACAT2-144H
Product Overview : Human ACAT2 partial ORF ( NP_005882, 104 a.a. - 213 a.a.) recombinant protein with GST-tag at N-terminal.
  • Specification
  • Gene Information
  • Related Products
Description : The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014]
Source : Wheat Germ
Species : Human
Tag : GST
Molecular Mass : 37.84 kDa
AA Sequence : QSIGIGDSSIVVAGGMENMSKAPHL AYLRTGVKIGEMPLTDSILCDGLTD AFHNCHMGITAENVATKWQVSREDQ DKVAVLSQNRTENAQKAGHFDKEIV PVLVSTRKGL
Applications : Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
Notes : Best use within three months from the date of receipt of this protein.
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Gene Name : ACAT2 acetyl-CoA acetyltransferase 2 [ Homo sapiens ]
Official Symbol : ACAT2
Synonyms : ACAT2; acetyl-CoA acetyltransferase 2; acetyl Coenzyme A acetyltransferase 2 , acetyl Coenzyme A acetyltransferase 2 (acetoacetyl Coenzyme A thiolase); acetyl-CoA acetyltransferase, cytosolic; acetoacetyl Coenzyme A thiolase; cytosolic acetoacetyl-CoA thiolase; acetyl-CoA transferase-like protein;
Gene ID : 39
mRNA Refseq : NM_005891
Protein Refseq : NP_005882
MIM : 100678
UniProt ID : Q9BWD1

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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How to measure the purity of recombinant ACAT2 protein? 02/05/2023

Typically, protein purity is measured using techniques such as SDS-PAGE, Western blotting, or HPLC.

Is there any discount for recombinant human ACAT2 protein (ACAT2-144H)? 12/23/2022

We are offering a 10% discount on all products for a limited time. The clock is ticking down. Be sure to take advantage of our holiday promotions!

How is the quality of recombinant ACAT2 protein ensured? 12/12/2022

Creative BioMart uses a variety of quality control measures to ensure the purity, identity, and activity of our products. These may include analytical testing such as SDS-PAGE, Western blotting, and ELISA, as well as functional assays to confirm activity. We can also perform endotoxin testing or use other methods to ensure the product is free from contaminants.

Are there any precautions or safety concerns to be aware of when working with recombinant ACAT2 protein? 03/14/2022

Like all proteins, recombinant ACAT2 protein should be handled with appropriate safety precautions, such as wearing gloves and avoiding direct contact with skin or mucous membranes. It is also important to follow good laboratory practices and to dispose of any waste materials properly.

What is the recommended concentration and volume of recombinant ACAT2 protein for use in assays or experiments? 02/21/2022

The recommended concentration and volume of recombinant ACAT2 protein can vary depending on the specific assay or experiment being performed. Typically, the protein is dissolved in a buffer solution at a desired concentration (e.g. 0.1-1 mg/mL) and then used as needed. It is important to ensure that the protein concentration is appropriate for the intended use, as high concentrations may result in nonspecific binding or other issues.

What are the potential applications of recombinant ACAT2 protein? 12/12/2021

Recombinant ACAT2 protein can be used in a variety of research applications, including studying the function of ACAT2 in lipid metabolism and cholesterol homeostasis, as well as in drug discovery efforts related to cardiovascular disease, obesity, and other metabolic disorders. It may also be used as a reference or standard in diagnostic tests for certain diseases.

How are the expression and activity of ACAT2 regulated in the body? 08/22/2021

The expression and activity of ACAT2 are regulated by a variety of factors, including dietary cholesterol intake, hormonal signals, and transcriptional and post-transcriptional mechanisms. Studies have identified several transcription factors that bind to the ACAT2 promoter region and regulate its expression, as well as post-transcriptional mechanisms such as alternative splicing and miRNA-mediated regulation.

Are there any alternative sources of ACAT2 protein, such as natural sources? 03/17/2021

In addition to recombinant ACAT2 protein, there may be natural sources of ACAT2 protein, such as from animal or human tissues. However, these sources may be more difficult to obtain and may have variable purity or activity. It is important to evaluate the quality and activity of these alternative sources before use in experiments or assays.

What are the potential therapeutic applications of targeting ACAT2? 10/12/2020

Inhibition of ACAT2 has been proposed as a potential therapeutic strategy for the treatment of cardiovascular disease, as well as other metabolic disorders such as nonalcoholic fatty liver disease (NAFLD) and type 2 diabetes. Several small molecule inhibitors of ACAT2 have been developed and are currently undergoing preclinical and clinical evaluation.

What is the role of ACAT2 in lipid metabolism and its association with atherosclerosis? 09/18/2020

ACAT2 is a key enzyme involved in the conversion of free cholesterol to cholesterol esters, which are stored in lipid droplets in cells. Dysregulation of ACAT2 activity has been implicated in the development of atherosclerosis and other metabolic disorders. Studies have shown that inhibition of ACAT2 can reduce atherosclerotic plaque formation in animal models, suggesting that ACAT2 may be a potential therapeutic target for the prevention and treatment of cardiovascular disease.

Customer Reviews (3)

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Reviews
01/02/2023

    The purity and quality of Creative BioMart's recombinant human ACAT2 protein is unmatched. I have tried other protein products in the past, but this one is by far the best.

    12/12/2022

      The recombinant human ACAT2 protein is of high quality and purity, and it has worked perfectly in my experiments.

      12/12/2021

        The recombinant human ACAT2 protein from Creative BioMart is highly pure and stable, and I have had great success using it in my assays.

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