Recombinant Human ACAT2, T7-tagged

• Cat.No.: ACAT2-29H
• Product Overview: Recombinant Human Acetyl-Coenzyme A acetyltransferase 2 produced inE.Coliis a non-glycosylated, polypeptide chain containing amino acids 1-397 and having a total molecular mass of 41.3 kda. ACAT2 contains T7 tag at N-terminus. ACAT-2 is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: T7
• Protein Length: 1-397 a.a.
• Description: Acetyl-Coenzyme A acetyltransferase 2 is an enzyme involved in lipid metabolism. Reported patients with ACAT2 deficiency have shown severe mental retardation and hypotonus. The ACAT2 gene shows complementary overlapping with the 3 prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.
• Physical Appearance: Sterile Filtered clear solution (0.77 mg/ml).
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Formulation: Acetyl-CoA acetyltransferase cytosolic at a concentration of 0.1 mg/ml in 10 mM Tris, pH 8.0, 0.1% Triton X-100 and 0.002% NaN3.
• Applications: • ELISA • MS • Inhibition Assays • Western Blotting.
• Stability: ACAT2 although stable at 14°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: ACAT2 acetyl-Coenzyme A acetyltransferase 2 [ Homo sapiens ]
• Synonyms: ACAT2; acetyl-Coenzyme A acetyltransferase 2;Acetyl-CoA acetyltransferase, cytosolic;Cytosolic acetoacetyl-CoA thiolase; Acetyl-CoA transferase-like protein; EC 2.3.1.9; OTTHUMP00000017527; Cytosolic acetoacetyl-CoA thiolase; acetoacetyl Coenzyme A thiolase; acetyl-Coenzyme A acetyltransferase 2; acetyl-Coenzyme A acetyltransferase 2 (acetoacetyl Coenzyme A thiolase)
• Gene ID: 39
• mRNA Refseq: NM_005891
• Protein Refseq: NP_005882
• UniProt ID: Q9BWD1
• Chromosome Location: 6q25.3
• MIM: 100678
• Pathway: Benzoate degradation via CoA ligation; Butanoate metabolism; Fatty acid metabolism; Lysine degradation; Metabolic pathways; Propanoate metabolism; Pyruvate metabolism; Synthesis and degradation of ketone bodies; Terpenoid backbone biosynthesis; Tryptophan metabolism; Valine, leucine and isoleucine degradation
• Function: acetyl-CoA C-acetyltransferase activity; acyltransferase activity; protein binding; transferase activity