Active Recombinant Human ACE2 protein, mFc-tagged

• Cat.No.: ACE2-185H
• Product Overview: Recombinant Human ACE2 protein(Gln 18 - Ser 740), fused with mFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: mFc
• Protein Length: Gln 18 - Ser 740
• Form: Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, Arginine, pH7.5 with glycerol as protectant.
• Bio-activity: Immobilized SARS-CoV-2 S protein RBD, His Tag at 1 μg/mL (100 μL/well) can bind Human ACE2, Mouse IgG1 Fc Tag with a linear range of 0.4-6 ng/mL.Immobilized Human ACE2, Mouse IgG1 Fc Tag at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 S1 protein, Fc Tag with a linear range of 1-20 ng/mL.
• Molecular Mass: This protein carries a mouse IgG1 Fc tag at the C-terminusThe protein has a calculated MW of 109.8 kDa. The protein migrates as 125-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  A high-throughput cell- and virus-free assay shows reduced neutralization of SARS-CoV-2 variants by COVID-19 convalescent plasma (2021)

Gene Information

• Gene Name: ACE2 angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 [ Homo sapiens ]
• Official Symbol: ACE2
• Synonyms: ACE2; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin-converting enzyme 2; metalloprotease MPROT15; ACE-related carboxypeptidase; angiotensin I converting enzyme 2; angiotensin-converting enzyme homolog; ACEH
• Gene ID: 59272
• mRNA Refseq: NM_021804
• Protein Refseq: NP_068576
• MIM: 300335
• UniProt ID: Q9BYF1