Active Recombinant Human ACE2 protein, hFc-tagged, Endotoxin-Free

• Cat.No.: ACE2-092H
• Product Overview: Active Recombinant Human ACE2 protein(Met 1-Ser 740)(NP_068576.1) was expressed in HEK293 with the fused Fc region of human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 1-740 aa
• Form: Supplied as sterile PBS, pH 7.4.
• Bio-activity: 1.Loaded Recombinant SARS-CoV-2 Spike RBD-mFc Recombinant Protein (HPLC-verified) on AMC Biosensor, can bind Recombinant Human ACE2 / Angiotensin-Converting Enzyme 2 Protein (Fc Tag) with an affinity constant of 1.36 nM as determined in BLI assay (Sartorius Octet RED384). 2.Recombinant Human ACE2 / Angiotensin-Converting Enzyme 2 Protein (Fc Tag) captured on Protein A chip, can bind Recombinant SARS-CoV-2 B.1.1.529 (Omicron) Spike S1 Protein (His Tag) with an affinity constant of 9.621 nM as determined in an SPR assay (Biacore T200).
• Molecular Mass: The recombinant human ACE2/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 961 amino acids and predicts a molecular mass of 110.3 kDa. As a result of glycosylation, the band migrates approximately 140-150 kDa in the SDS-PAGE under reducing conditions.
• Endotoxin: < 0.5 EU per mg protein.
• Purity: ≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
• Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Concentration: 0.1-1.0 mg/mL. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ACE2 angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 [ Homo sapiens ]
• Official Symbol: ACE2
• Synonyms: ACE2; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin-converting enzyme 2; metalloprotease MPROT15; ACE-related carboxypeptidase; angiotensin I converting enzyme 2; angiotensin-converting enzyme homolog; ACEH
• Gene ID: 59272
• mRNA Refseq: NM_021804
• Protein Refseq: NP_068576
• MIM: 300335
• UniProt ID: Q9BYF1