Recombinant Mouse Ace2 protein, His&hFc-tagged

• Cat.No.: Ace2-1183M
• Product Overview: Recombinant Mouse Ace2 protein(Q8R0I0-1)(Met1-Thr740), fused with His&hFc tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc&His
• Protein Length: Met1-Thr740
• Form: Lyophilized from sterile 20mM Tris, 150mM NaCl, 1mM ZnCl2, pH 7.5Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Molecular Mass: The secreted recombinant mouse ACE2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 971 amino acids and has a predicted molecular mass of 112 kDa. As a result of glycosylation, it migrates as an approximately 130 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  A multiplexed high-throughput neutralization assay reveals a lack of activity against multiple variants after SARS-CoV-2 infection (2021)
  CXCL12 and CXCL13 Cytokine Serum Levels Are Associated with the Magnitude and the Quality of SARS-CoV-2 Humoral Responses (2022)

Gene Information

• Gene Name: Ace2 angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 [ Mus musculus ]
• Official Symbol: Ace2
• Synonyms: ACE2; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin-converting enzyme 2; ACE-related carboxypeptidase; angiotensin I converting enzyme 2; 2010305L05Rik
• Gene ID: 70008
• mRNA Refseq: NM_001130513
• Protein Refseq: NP_001123985