Recombinant Human ACOT1 Protein, MYC/DDK-tagged, C13 and N15-labeled

• Cat.No.: ACOT1-323H
• Product Overview: ACOT1 MS Standard C13 and N15-labeled recombinant protein (NP_001032238) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating intracellular levels of acyl-CoAs, free fatty acids and CoASH. More active towards saturated and unsaturated long chain fatty acyl-CoAs (C12-C20).
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 46.1 kDa
• AA Sequence: MAATLILEPAGRCCWDEPVRIAVRG LAPEQPVTLRASLRDEKGALFQAHA RYRADTLGELDLERAPALGGSFAGL EPMGLLWALEPEKPLVRLVKRDVRT PLAVELEVLDGHDPDPGRLLCRVRH ERYFLPPGVRREPVRAGRVRGTLFL PPEPGPFPGIVDMFGTGGGLLEYRA SLLAGKGFAVMALAYYNYEDLPKTM ETLHLEYFEEAVNYLLSHPEVKGPG VGLLGISKGGELCLSMASFLKGITA AVVINGSVANVGGTLRYKGETLPPV GVNRNRIKVTKDGYADIVDVLNSPL EGPDQKSFIPVERAESTFLFLVGQD DHNWKSEFYANEACKRLQAHGRRKP QIICYPETGHYIEPPYFPLCRASLH ALVGSPIIWGGEPRAHAMAQVDAWK QLQTFFHKHLGGHEGTIPSKVTRTR PLEQKLISEEDLAANDILDYKDDDD KV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ACOT1 acyl-CoA thioesterase 1 [ Homo sapiens (human) ]
• Official Symbol: ACOT1
• Synonyms: ACOT1; acyl-CoA thioesterase 1; acyl-coenzyme A thioesterase 1; ACH2; CTE 1; LACH2; CTE-I; CTE-Ib; long chain acyl-CoA hydrolase; long chain acyl-CoA thioester hydrolase; inducible cytosolic acyl-coenzyme A thioester hydrolase; CTE-1
• Gene ID: 641371
• mRNA Refseq: NM_001037161
• Protein Refseq: NP_001032238
• MIM: 614313
• UniProt ID: Q86TX2