Recombinant Human ACTB Protein, His-tagged

• Cat.No.: ACTB-124H
• Product Overview: Recombinant Human ACTB Protein with His tag was expressed in E. coli.

Specification

• Description: Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are ubiquitously expressed, controlling cell structure and motility. While all actin isoforms are highly homologous, cytoplasmic β- and γ-actin protein sequences differ by only four biochemically similar amino acids. For this reason, antibodies raised to β-actin may cross-react with γ-actin, and vice versa. α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells. Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin. The ARP2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments. Research studies have shown that actin is hyperphosphorylated in primary breast tumors. Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle, as shown in research studies. Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome-dependent muscle proteolysis.
• Source: E. coli
• Species: Human
• Tag: His
• Molecular Mass: ~43 kDa
• AA Sequence: MDDDIAALVVDNGSGMCKAGFAGDD APRAVFPSIVGRPRHQGVMVGMGQK DSYVGDEAQSKRGILTLKYPIEHGI VTNWDDMEKIWHHTFYNELRVAPEE HPVLLTEAPLNPKANREKMTQIMFE TFNTPAMYVAIQAVLSLYASGRTTG IVMDSGDGVTHTVPIYEGYALPHAI LRLDLAGRDLTDYLMKILTERGYSF TTTAEREIVRDIKEKLCYVALDFEQ EMATAASSSSLEKSYELPDGQVITI GNERFRCPEALFQPSFLGMESCGIH ETTFNSIMKCDVDIRKDLYANTVLS GGTTMYPGIADRMQKEITALAPSTM KIKIIAPPERKYSVWIGGSILASLS TFQQMWISKQEYDESGPSIVHRKCF
• Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).
• Notes: For research use only, not for use in diagnostic procedure.
• Storage: Store at 4 centigrade short term. Aliquot and store at -20 centigrade long term. Avoid freeze-thaw cycles.
• Storage Buffer: PBS, pH7.4

Gene Information

• Gene Name: ACTB actin, beta [ Homo sapiens (human) ]
• Official Symbol: ACTB
• Synonyms: ACTB; actin, beta; actin, cytoplasmic 1; beta cytoskeletal actin; PS1TP5-binding protein 1; PS1TP5BP1
• Gene ID: 60
• mRNA Refseq: NM_001101
• Protein Refseq: NP_001092
• MIM: 102630
• UniProt ID: P60709

Reviews

08/19/2022

I could trust the product's precision, ensuring reliable data generation

08/15/2021

The protein product had an impressive shelf life, maintaining its quality and functionality over an extended period

03/04/2021

The protein product seamlessly integrated with various experimental techniques, enhancing its versatility

02/23/2020

The protein product underwent rigorous safety testing, ensuring its reliability and suitability for experimentation

06/19/2018

The protein product line offered a wide variety of options for different research need

Q&As

Which biological process GO terms were identified through the PPI analysis?

 The PPI analysis identified several biological process GO terms such as "Actin cytoskeleton organization," "Box C/D snoRNP assembly," "Actin filament depolymerization," "Cell junction assembly," and "DNA repair."

What was the focus of the current study mentioned?

The current study aimed to analyze the diagnostic value of ACTB and predict its independent prognostic factor in distinct cancer subtypes using detailed in silico analysis.

How could ACTB potentially serve as a biomarker in LIHC, HNSC, and LUAD?

The findings suggest that due to its elevated expression, association with poorer survival and metastasis, and correlation with important parameters such as immune infiltration and tumor purity, ACTB has the potential to be a candidate biomarker for LIHC, HNSC, and LUAD.

How is ACTB related to the metastasis of different cancers?

The dysregulation and polymerization of ACTB have been revealed to be associated with the metastasis of different cancer types.

Which drugs were found to reduce the expression level of ACTB?

Warfarin and aflatoxin B1 were found to potentially reduce the expression level of ACTB.

How many highly significant transcription factors (TFs) were identified as potential regulators of ACTB expression?

Ten highly significant TFs were identified as potential regulators of ACTB expression. They are AATF, WWTR1, GFI1, NR3C1, MYC, GFI1, STAT3, ING1, MAX, and POU4F1.

What do the results suggest about the regulation of ACTB expression?

The results indicate that a variety of different regulators, including miRNAs and TFs, are involved in the regulation of ACTB expression.

What analytical method was used to identify potential regulators of ACTB expression?

Enrichr analysis was used to identify potential regulators of ACTB expression.

What molecular functions were associated with the ACTB-associated genes?

The molecular functions associated with the ACTB-associated genes included "Actin monomer binding," "ATPase activity," "Leucine zipper domain binding," and "DNA helicase activity."

Which drugs were found to elevate the expression level of ACTB?

Valporic acid and metribolone were found to potentially elevate the expression level of ACTB.

What is the significance of ACTB (eta-actin) as a housekeeping gene?

ACTB serves as a housekeeping gene commonly used as a control in measuring gene expression in various diseases.

How many miRNAs were found to have the potential to change ACTB expression?

Ten miRNAs were identified as potential regulators of ACTB expression. They are hsa-miR-1908, hsa-miR-663, hsa-miR-744, hsa-miR-4745-3p, hsa-miR-1538, hsa-miR-3960, hsa-miR-4749-5p, hsa-miR-4706, hsa-miR-4743, and hsa-miR-3180-3p.

In which specific cancers was elevated ACTB expression associated with poorer survival and metastasis?

Elevated ACTB expression was associated with poorer survival and metastasis specifically in liver hepatocellular carcinoma (LIHC), head and neck squamous cancer (HNSC), and lung adenocarcinoma (LUAD).

What did the protein-protein interaction (PPI) analysis reveal about ACTB-associated genes?

The PPI analysis revealed a set of ten ACTB-associated genes that are involved in various biological processes, including "Actin cytoskeleton organization," "Box C/D snoRNP assembly," "Actin filament depolymerization," "Cell junction assembly," and "DNA repair."

What analytical approach was used in the study?

The study utilized in silico analysis to assess the diagnostic value and independent prognostic factor of ACTB in different cancer subtypes.

How was ACTB expression influenced through CTD analysis?

Through CTD analysis, it was observed that ACTB expression can potentially be influenced by a number of drugs.

Which cancers have been found to have a close association with ACTB expression?

 ACTB expression has been found to be closely related to various cancers, including liver, pancreatic, renal, colorectal, melanoma, prostate, esophageal, lung, gastric, breast, and ovarian cancers.

How was ACTB-associated expression regulated in the study?

The study explored ACTB-associated clinically important expression regulators, including TFs (transcription factors), miRNAs, and different chemotherapeutic drugs.

What was observed regarding ACTB expression in major human cancer tissues compared to normal samples?

ACTB expression was found to be remarkably higher in 24 major human cancer tissues compared to normal samples.

What other parameters did ACTB up-regulation show interesting correlations with?

ACTB up-regulation showed interesting correlations with immune infiltration of CD4+ T and CD8+ T cells, tumor purity, mutant genes, and other important parameters.