Recombinant Human ACTC1 protein, His & T7-tagged

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Recombinant Human ACTC1 protein, His & T7-tagged

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Cat.No. : ACTC1-6835H
Product Overview : Recombinant Human ACTC1 aa. (Asp156~Gly368 (Accession # P68032)) fused with N-terminal His & T7 tag was produced in E. coli cells.
Description : Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC).
Source : E. coli
Species : Human
Tag : His & T7
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 27.6kDa.
Protein length : Asp156~Gly368 (Accession # P68032)
Endotoxin : <1.0EU per 1ug (determined by the LAL method)
Purity : >95%
Characteristic : The isoelectric point is 5.7.
Applications : SDS-PAGE; WB; ELISA; IP.
Stability : The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage buffer : Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4.
SDS-PAGE :

Gene Name : ACTC1 actin, alpha, cardiac muscle 1 [ Homo sapiens (human) ]
Official Symbol : ACTC1
Synonyms : ACTC; ASD5; CMD1R; CMH11; LVNC4; actin, alpha cardiac muscle 1
Gene ID : 70
mRNA Refseq : NM_005159.4
Protein Refseq : NP_005150.1
UniProt ID : P68032

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