Recombinant Human ACTN1 Protein, GST-tagged

• Cat.No.: ACTN1-228H
• Product Overview: Human ACTN1 full-length ORF ( NP_001093.1, 1 a.a. - 892 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 129.5 kDa
• AA Sequence: MDHYDSQQTNDYMQPEEDWDRDLLL DPAWEKQQRKTFTAWCNSHLRKAGT QIENIEEDFRDGLKLMLLLEVISGE RLAKPERGKMRVHKISNVNKALDFI ASKGVKLVSIGAEEIVDGNVKMTLG MIWTIILRFAIQDISVEETSAKEGL LLWCQRKTAPYKNVNIQNFHISWKD GLGFCALIHRHRPELIDYGKLRKDD PLTNLNTAFDVAEKYLDIPKMLDAE DIVGTARPDEKAIMTYVSSFYHAFS GAQKAETAANRICKVLAVNQENEQL MEDYEKLASDLLEWIRRTIPWLENR VPENTMHAMQQKLEDFRDYRRLHKP PKVQEKCQLEINFNTLQTKLRLSNR PAFMPSEGRMVSDINNAWGCLEQVE KGYEEWLLNEIRRLERLDHLAEKFR QKASIHEAWTDGKEAMLRQKDYETA TLSEIKALLKKHEAFESDLAAHQDR VEQIAAIAQELNELDYYDSPSVNAR CQKICDQWDNLGALTQKRREALERT EKLLETIDQLYLEYAKRAAPFNNWM EGAMEDLQDTFIVHTIEEIQGLTTA HEQFKATLPDADKERLAILGIHNEV SKIVQTYHVNMAGTNPYTTITPQEI NGKWDHVRQLVPRRDQALTEEHARQ QHNERLRKQFGAQANVIGPWIQTKM EEIGRISIEMHGTLEDQLSHLRQYE KSIVNYKPKIDQLEGDHQLIQEALI FDNKHTNYTMEHIRVGWEQLLTTIA RTINEVENQILTRDAKGISQEQMNE FRASFNHFDRDHSGTLGPEEFKACL ISLGYDIGNDPQGEAEFARIMSIVD PNRLGVVTFQAFIDFMSRETADTDT ADQVMASFKILAGDKNYITMDELRR ELPPDQAEYCIARMAPYTGPDSVPG ALDYMSFSTALYGESDL
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ACTN1 actinin, alpha 1 [ Homo sapiens ]
• Official Symbol: ACTN1
• Synonyms: ACTN1; actinin, alpha 1; alpha-actinin-1; actinin 1 smooth muscle; non-muscle alpha-actinin-1; F-actin cross-linking protein; alpha-actinin cytoskeletal isoform; FLJ40884; FLJ54432
• Gene ID: 87
• mRNA Refseq: NM_001102
• Protein Refseq: NP_001093
• MIM: 102575
• UniProt ID: P12814