| Description : |
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. |
| Source : |
Mammalian cells |
| Species : |
Human |
| Tag : |
Flag |
| Form : |
25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
| Molecular Mass : |
102.9 kDa |
| AA Sequence : |
MDHYDSQQTNDYMQPEEDWDRDLLL DPAWEKQQRKTFTAWCNSHLRKAGT QIENIEEDFRDGLKLMLLLE VISGERLAKPERGKMRVHKISNVNK ALDFIASKGVKLVSIGAEEIVDGNV KMTLGMIWTIILRFAIQDIS VEETSAKEGLLLWCQRKTAPYKNVN IQNFHISWKDGLGFCALIHRHRPEL IDYGKLRKDDPLTNLNTAFD VAEKYLDIPKMLDAEDIVGTARPDE KAIMTYVSSFYHAFSGAQKAETAAN RICKVLAVNQENEQLMEDYE KLASDLLEWIRRTIPWLENRVPENT MHAMQQKLEDFRDYRRLHKPPKVQE KCQLEINFNTLQTKLRLSNR PAFMPSEGRMVSDINNAWGCLEQVE KGYEEWLLNEIRRLERLDHLAEKFR QKASIHEAWTDGKEAMLRQK DYETATLSEIKALLKKHEAFESDLA AHQDRVEQIAAIAQELNELDYYDSP SVNARCQKICDQWDNLGALT QKRREALERTEKLLETIDQLYLEYA KRAAPFNNWMEGAMEDLQDTFIVHT IEEIQGLTTAHEQFKATLPD ADKERLAILGIHNEVSKIVQTYHVN MAGTNPYTTITPQEINGKWDHVRQL VPRRDQALTEEHARQQHNER LRKQFGAQANVIGPWIQTKMEEIGR ISIEMHGTLEDQLSHLRQYEKSIVN YKPKIDQLEGDHQLIQEALI FDNKHTNYTMEHIRVGWEQLLTTIA RTINEVENQILTRDAKGISQEQMNE FRASFNHFDRDHSGTLGPEE FKACLISLGYDIGNDPQGEAEFARI MSIVDPNRLGVVTFQAFIDFMSRET ADTDTADQVMASFKILAGDK
NYITMDELRRELPPDQAEYCIARMA PYTGPDSVPGALDYMSFSTALYGES DLTRTRPLEQKLISEEDLAANDILD YKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining. |
| Stability : |
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Storage : |
Store at -80 centigrade. |
| Concentration : |
>50 ug/mL as determined by microplate BCA method. |
| Preparation : |
Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Protein Families : |
Druggable Genome, ES Cell Differentiation/IPS |
| Protein Pathways : |
Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Systemic lupus erythematosus, Tight junction |
