Recombinant Human ACTN1 protein, His-tagged

• Cat.No.: ACTN1-45H
• Product Overview: Recombinant human ACTN1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: ACTN1 also known as alpha-actinin-1 isoform a. ACTN1 functions as an actin-binding-protein. ACTN1 determines the motility of keratinocytes by regulating the organization of the actin cytoskeleton, focal adhesion, and hemidesmosome proteins complexes, thereby modulating cell speed, lamellipodial dynamics, and directed migration.
• Form: Liquid. In Phosphate Buffered Saline (pH7.4) containing 10% glycerol
• Molecular Mass: 31.4kDa (274aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSEFMDHYD SQQTNDYMQP EEDWDRDLLL DPAWEKQQRK TFTAWCNSHL RKAGTQIENI EEDFRDGLKLMLLLEVISGE RLAKPERGKM RVHKISNVNK ALDFIASKGV KLVSIGAEEI VDGNVKMTLG MIWTIILRFA IQDISVEETS AKEGLLLWCQRKTAPYKNVN IQNFHISWKD GLGFCALIHR HRPELIDYGK LRKDDPLTNL NTAFDVAEKY LDIPKMLDAE DIVGTARPDE KAIMTYVSSFYHAF
• Purity: >95% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: ACTN1 actinin, alpha 1 [ Homo sapiens ]
• Official Symbol: ACTN1
• Synonyms: ACTN1; actinin, alpha 1; alpha-actinin-1; actinin 1 smooth muscle; non-muscle alpha-actinin-1; F-actin cross-linking protein; alpha-actinin cytoskeletal isoform; FLJ40884; FLJ54432
• Gene ID: 87
• mRNA Refseq: NM_001102
• Protein Refseq: NP_001093
• MIM: 102575
• UniProt ID: P12814
• Chromosome Location: 14q24.1
• Pathway: Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Arrhythmogenic right ventricular cardiomyopathy (ARVC), organism-specific biosystem; Arrhythmogenic right ventricular cardiomyopathy (ARVC), conserved biosystem; Cell junction organization, organism-specific biosystem
• Function: actin binding; actin filament binding; calcium ion binding; integrin binding; ion channel binding; protein binding; protein domain specific binding; vinculin binding