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Recombinant Human ADD3 Protein, GST-tagged

Cat.No. : ADD3-339H
Product Overview : Human ADD3 partial ORF ( NP_058432, 462 a.a. - 560 a.a.) recombinant protein with GST-tag at N-terminal.
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  • Gene Information
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Description : Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced adducin gamma transcripts encoding different isoforms have been described. The functions of the different isoforms are not known. [provided by RefSeq, Jul 2008]
Source : Wheat Germ
Species : Human
Tag : GST
Molecular Mass : 36.63 kDa
AA Sequence : PRTKITWMKAEDSSKVSGGTPIKIE DPNQFVPLNTNPNEVLEKRNKIREQ NRYDLKTAGPQSQLLAGIVVDKPPS TMQFEDDDHGPPAPPNPFSHLTEG
Applications : Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
Notes : Best use within three months from the date of receipt of this protein.
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Gene Name : ADD3 adducin 3 (gamma) [ Homo sapiens ]
Official Symbol : ADD3
Synonyms : ADDL
Gene ID : 120
mRNA Refseq : NM_019903.3
Protein Refseq : NP_063968.1
MIM : 601568
UniProt ID : Q9UEY8

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What are the consequences of modulating the expression or activity of ADD3 on cellular processes like cell adhesion, cytoskeletal organization, or membrane dynamics? 08/07/2022

Modulating the expression or activity of ADD3 can impact cellular processes like cell adhesion, cytoskeletal organization, or membrane dynamics.

What are the downstream signaling pathways or target molecules regulated by ADD3, and how do they contribute to its cellular functions? 10/04/2021

ADD3 regulates downstream signaling pathways or target molecules that contribute to its cellular functions.

What is the subcellular localization of ADD3 within cells? Does it exhibit any dynamic localization or translocation? 12/30/2020

ADD3 exhibits subcellular localization, and its localization may undergo dynamic changes or translocation.

Can the functional domains or motifs within ADD3 be identified and characterized? 12/24/2020

Functional domains or motifs within ADD3 can be identified and characterized.

What post-translational modifications, such as phosphorylation or acetylation, occur on ADD3 and how do they impact its function? 03/31/2020

Post-translational modifications, such as phosphorylation or acetylation, occur on ADD3 and can influence its function.

Are there specific interacting proteins or binding partners that associate with ADD3 in a cell- or context-specific manner? 03/16/2020

ADD3 interacts with specific proteins or binding partners, and these interactions can be context-dependent.

How is the expression of ADD3 regulated during development or in response to specific stimuli? 04/29/2018

The expression of ADD3 can be regulated during development or in response to specific stimuli.

Can specific antibodies or probes be developed to visualize the localization or dynamics of ADD3 in live cells or tissues? 02/15/2018

Specific antibodies or probes can be developed to visualize the localization or dynamics of ADD3 in live cells or tissues.

Are there any small molecules or drugs that can selectively modulate the activity or stability of ADD3? 03/09/2017

There may exist small molecules or drugs that selectively modulate the activity or stability of ADD3.

What is the expression pattern of ADD3 in different tissues and cell types? 05/15/2016

The expression pattern of ADD3 varies across different tissues and cell types.

Customer Reviews (2)

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Reviews
03/06/2021

    Shows excellent performance in proximity ligation assays for protein-protein interaction studies.

    07/25/2020

      Performs exceptionally well in protein crystallization experiments, improving crystal quality and yield.

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