Recombinant Human ADSSL1 293 Cell Lysate

• Cat.No.: ADSSL1-8993HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for adenylosuccinate synthase like 1 (ADSSL1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ADSSL1 adenylosuccinate synthase like 1 [ Homo sapiens ]
• Official Symbol: ADSSL1
• Synonyms: ADSSL1; adenylosuccinate synthase like 1; adenylosuccinate synthetase isozyme 1; FLJ38602; adSS 1; AMPSase 1; IMP--aspartate ligase 1; M-type adenylosuccinate synthetase; adenylosuccinate synthetase, basic isozyme; adenylosuccinate synthetase, muscle isozyme
• Gene ID: 122622
• mRNA Refseq: NM_152328
• Protein Refseq: NP_689541
• MIM: 612498
• UniProt ID: Q8N142
• Chromosome Location: 14q32.33
• Pathway: Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of nucleotides, organism-specific biosystem; Purine metabolism, organism-specific biosystem; Purine metabolism, organism-specific biosystem
• Function: GTP binding; adenylosuccinate synthase activity; ligase activity; magnesium ion binding; nucleotide binding; phosphate ion binding