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Recombinant Human AFP protein, M2 Peptide fused

Cat.No.: AFP-29H
Product Overview: Recombinant Human AFP fusion protein was constructed by fusion human alpha fetal protein (AFP) N-terminal domain-I (1-198 aa) with human collagen type I derived AAQIRSQVMTHLRVIYER peptide and was expressed in E. coli.
Description: Cell adhesion to the extracellular matrix (ECM) is essential for the development and maintenance of cells. Cell adhesion to various ECM as ligands is mainly mediated by cell surface receptor such as integrins. Human Collagen Type I is a key component in ECM for cell attachment and differentiation. M2 Peptide AAQIRSQVMTHLRVIYER that derived from human collagen type I protein has been demonstrated in vitro as collagen mimetics and specific interacts with APP. This peptide will be a good coating matrix for cell attachment in serum free medium or enhance specific cell lineage differentiation.
Source: E. coli
Species: Human
Form: 1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol.
Purity: >90% by SDS-PAGE
Applications: 1. As fusion protein fully derived from human protein, can be used as coating matrix protein to replace human collagen type I for human cell differentiation regulation study in vitro.2. Can be used in low serum or serum-free cell culture media to improve attachment and spreading of many normal and stem cells including human ES cell, endothelial, epithelial, hepatocytes and mesenchymal stem cells.3. Can be used to improve survival of primary cultures.
Storage: Keep at -80centigrade for long term storage. Product is stable at 4 centigrade for at least 30 days.
Gene Name: AFP alpha-fetoprotein [ Homo sapiens ]
Official Symbol: AFP
Synonyms: AFP; alpha-fetoprotein; HPAFP; FETA; alpha-fetoglobulin; alpha-1-fetoprotein;
Gene ID: 174
mRNA Refseq: NM_001134
Protein Refseq: NP_001125
MIM:
UniProt ID: P02771
Chromosome Location: 4q11-q13
Pathway: Direct p53 effectors, organism-specific biosystem; FOXA2 and FOXA3 transcription factor networks, organism-specific biosystem; Glucocorticoid receptor regulatory network, organism-specific biosystem;
Function: metal ion binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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