Recombinant Human AFP Protein (Met1-Val609), HlgG1 Fc-tagged

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Recombinant Human AFP Protein (Met1-Val609), HlgG1 Fc-tagged

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Cat.No. : AFP-1059H
Product Overview : Recombinant Human AFP Protein (NP_001125.1) (Met1-Val609) was produced by HEK293 Cells expression system. This protein was expressed with the Fc region of human IgG1 at the C-terminus.
Description : Alpha-fetoprotein (AFP) is classified as a member of the albuminoid gene superfamily consisting of albumin, AFP, vitamin D (Gc) protein, and alpha-albumin. AFP is a glycoprotein of 591 amino acids and a carbohydrate moiety. AFP is one of the several embryo-specific proteins and is a dominant serum protein as early in human embryonic life as one month, when albumin and transferrin are present in relatively small amounts. It is first synthesized in the human by the yolk sac and liver(1-2 months) and subsequently predominantly in the liver. A small amount of AFP is produced by the GI tract of the human conceptus. It has been proved that AFP may reappear in the serum in elevated amounts in adult life in association with normal restorative processes and with malignant growth. Alpha-fetoprotein (AFP) is a specific marker for hepatocellular carcinoma (HCC), teratoblastomas, and neural tube defect (NTD).
Source : HEK293
Species : Human
Tag : HlgG1 Fc
Predicted N Terminal : Arg 19
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Molecular Mass : The recombinant human AFP consists of 832 amino acids and predicts a molecular mass of 93.5 kDa.
Protein length : Met1-Val609
Endotoxin : < 1.0 EU per μg protein as determined by the LAL method.
Purity : > 90 % as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -70 centigrade.
Storage : Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Shipping : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Gene Name : AFP alpha-fetoprotein [ Homo sapiens ]
Official Symbol : AFP
Synonyms : AFP; alpha-fetoprotein; HPAFP; FETA; alpha-fetoglobulin;
Gene ID : 174
mRNA Refseq : NM_001134
Protein Refseq : NP_001125
UniProt ID : P02771

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