Recombinant Human AGER protein

• Cat.No.: AGER-7323H
• Product Overview: Recombinant Human AGER protein(NP_001127.1) (Met1-Ala 344) was expressed in HEK293 with six amino acids (LEVLFQ) at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Protein Length: 1-344 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: 1. Measured by its ability to compete with Biotinylated recombinant human AGER for binding to immobilized recombinant human Fc-S100B in a functional ELISA. 2. Measured by its ability to compete with Biotinylated recombinant human AGER for binding to immobilized recombinant mouse S100B-Fc in a functional ELISA. 3. Measured by its ability to compete with Biotinylated recombinant human AGER for binding to immobilized recombinant human S100A1-Fc in a functional ELISA. 4. Measured by its ability to compete with Biotinylated recombinant human AGER for binding to immobilized recombinant human APP-Fc in a functional ELISA.
• Molecular Mass: The recombinant human AGER consists of 329 amino acids and predicts a molecular mass of 35 KDa. It migrates as an approximately 46-52 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  • Early expression of the receptor for advanced glycation end products in a toxic model produced by 6-hydroxydopamine in the rat striatum. (2016)

Gene Information

• Gene Name: AGER advanced glycosylation end product-specific receptor [ Homo sapiens ]
• Official Symbol: AGER
• Synonyms: AGER; advanced glycosylation end product-specific receptor; RAGE; RAGE isoform sRAGE-delta; RAGE isoform NtRAGE-delta
• Gene ID: 177
• mRNA Refseq: NM_001136
• Protein Refseq: NP_001127
• MIM: 600214
• UniProt ID: Q15109

Citation

Early expression of the receptor for advanced glycation end products in a toxic model produced by 6-hydroxydopamine in the rat striatum.

Journal: Chemico-biological interactions    PubMed ID: 26902637    Data: 2016/8/1

Authors: Iris N Serratos, Pilar Castellanos, Abel Santamaria

Article Snippet:NeuN (monoclonal mouse antibody, MAB377 Clone A60, 1:200) was purchased from EMD Millipore Co. (Billerica, MA, USA).NeuN (monoclonal mouse antibody, MAB377 Clone A60, 1:200) was purchased from EMD Millipore Co. (Billerica, MA, USA).. The recombinant human form of RAGE (also described in the catalogue as AGER-7323H) consisted of 322 amino acids and was obtained from Creative BioMart (NY, USA) .