Recombinant Human AKAP5 Protein, GST-tagged
Cat.No. : | AKAP5-401H |
Product Overview : | Human AKAP5 full-length ORF ( NP_004848.2, 1 a.a. - 427 a.a.) recombinant protein with GST-tag at N-terminal. |
- Specification
- Gene Information
- Related Products
Description : | The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to the RII-beta regulatory subunit of PKA, and also to protein kinase C and the phosphatase calcineurin. It is predominantly expressed in cerebral cortex and may anchor the PKA protein at postsynaptic densities (PSD) and be involved in the regulation of postsynaptic events. It is also expressed in T lymphocytes and may function to inhibit interleukin-2 transcription by disrupting calcineurin-dependent dephosphorylation of NFAT. [provided by RefSeq, Jul 2008] |
Source : | Wheat Germ |
Species : | Human |
Tag : | GST |
Molecular Mass : | 73.5 kDa |
AA Sequence : | METTISEIHVENKDEKRSAEGSPGA ERQKEKASMLCFKRRKKAAKALKPK AGSEAADVARKCPQEAGASDQPEPT RGAWASLKRLVTRRKRSESSKQQKP LEGEMQPAINAEDADLSKKKAKSRL KIPCIKFPRGPKRSNHSKIIEDSDC SIKVQEEAEILDIQTQTPLNDQATK AKSTQDLSEGISRKDGDEVCESNVS NSITSGEKVISVELGLDNGHSAIQT GTLILEEIETIKEKQDVQPQQASPL ETSETDHQQPVLSDVPPLPAIPDQQ IVEEASNSTLESAPNGKDYESTEIV AEETKPKDTELSQESDFKENGITEE KSKSEESKRMEPIAIIITDTEISEF DVTKSKNVPKQFLISAENEQVGVFA NDNGFEDRTSEQYETLLIETASSLV KNAIQLSIEQLVNEMASDDNKINNL LQ |
Applications : | Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array |
Notes : | Best use within three months from the date of receipt of this protein. |
Storage : | Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing. |
Storage Buffer : | 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer. |
Gene Name : | AKAP5 A kinase (PRKA) anchor protein 5 [ Homo sapiens ] |
Official Symbol : | AKAP5 |
Synonyms : | AKAP5; A kinase (PRKA) anchor protein 5; A-kinase anchor protein 5; AKAP75; AKAP79; AKAP-5; AKAP 79; A-kinase anchor protein 79 kDa; A-kinase anchor protein, 79kDa; A-kinase anchoring protein 75/79; cAMP-dependent protein kinase regulatory subunit II high affinity binding protein; cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein; H21; |
Gene ID : | 9495 |
mRNA Refseq : | NM_004857 |
Protein Refseq : | NP_004848 |
MIM : | 604688 |
UniProt ID : | P24588 |
Products Types
◆ Recombinant Protein | ||
AKAP5-2451H | Recombinant Human AKAP5 Protein, His (Fc)-Avi-tagged | +Inquiry |
AKAP5-431M | Recombinant Mouse AKAP5 Protein, His (Fc)-Avi-tagged | +Inquiry |
Akap5-110M | Recombinant Mouse Akap5 Protein, His&GST-tagged | +Inquiry |
AKAP5-714H | Recombinant Human AKAP5 | +Inquiry |
AKAP5-23H | Recombinant Human AKAP5, His-tagged | +Inquiry |
◆ Lysates | ||
AKAP5-8939HCL | Recombinant Human AKAP5 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (17)
Ask a questionAt present, AKAP5 is not established as a specific diagnostic marker for any diseases. However, ongoing research may uncover its potential diagnostic utility in certain conditions where AKAP5 dysregulation or altered expression has been observed.
AKAP5 contains multiple structural domains that contribute to its function. It has a long N-terminal region rich in potential protein-protein interaction sites, including amphipathic helices and an isoleucine-arginine motif. It also has a central domain that binds to the regulatory subunits of PKA and a C-terminal domain responsible for anchoring AKAP5 to various cellular structures.
Apart from PKA, AKAP5 interacts with multiple other molecules involved in signaling pathways. It can bind and recruit various enzymes, ion channels, receptors, and cytoskeletal proteins to specific subcellular locations. Some notable interacting partners of AKAP5 include phosphodiesterases, protein phosphatase 2B (calcineurin), NMDA receptors, L-type calcium channels, and adenylyl cyclases.
Yes, AKAP5 can interact with various isoforms of PKA by binding to their regulatory subunits. This allows for selective targeting of different PKA holoenzymes to specific subcellular locations.
Currently, there are no specific drugs or compounds that target AKAP5 directly. However, researchers are actively investigating the development of small molecules or peptides that can selectively disrupt AKAP5 interactions or modulate its functions.
AKAP5 plays a role in calcium signaling by anchoring PKA and calcium-related enzymes or channels. It is involved in regulating calcium-induced release of neurotransmitters and modulating NMDA receptor-mediated calcium influx.
Yes, AKAP5 knockout animal models have been generated to study its functions. These models have provided valuable insights into the role of AKAP5 in synaptic transmission, synaptic plasticity, and behavior.
Yes, AKAP5 can be phosphorylated by various kinases, including PKA itself. Phosphorylation of AKAP5 can regulate its localization, protein-protein interactions, and activity.
Some studies have identified genetic variations in AKAP5 that are associated with increased susceptibility to certain psychiatric disorders, including schizophrenia and bipolar disorder. However, more research is needed to fully understand the impact of these genetic variations on disease development and progression.
AKAP5 binds to the regulatory subunits of PKA, bringing the enzyme into close proximity with its substrate molecules. This interaction allows PKA to phosphorylate specific target proteins and regulate their function within the localized signaling microdomain.
Some studies highlight the involvement of AKAP5 in specific diseases and disorders. For instance, alterations in AKAP5 localization and function have been implicated in schizophrenia, bipolar disorder, and alcohol addiction. However, further research is necessary to establish a clearer understanding of AKAP5's role in these conditions.
Yes, the expression of AKAP5 can be regulated during development and in response to different physiological conditions or stimuli. It is known to be developmentally regulated in the brain, with changes in its expression levels during critical periods of synaptic maturation and plasticity.
While AKAP5's functions have been extensively studied in neurons, recent research suggests potential roles in other cell types. For example, AKAP5 has been implicated in cardiac function, where it regulates the localization and activity of ion channels and receptors. However, further investigation is needed to fully understand AKAP5's functions outside of the nervous system.
Yes, AKAP5 is important for synaptic plasticity, which is the ability of synapses to strengthen or weaken in response to neuronal activity. It anchors PKA and other signaling molecules to postsynaptic densities, where they modulate neurotransmitter receptor function, synaptic strength, and long-term potentiation (LTP) or long-term depression (LTD) processes associated with synaptic plasticity.
AKAP5 is widely expressed in various tissues, including the brain, heart, and skeletal muscle. It is especially abundant in neurons, where it is involved in regulating neuronal signaling and synaptic plasticity.
AKAP5 can be regulated through multiple mechanisms. One important regulatory mechanism involves phosphorylation, where various kinases can target AKAP5 and modulate its activity or binding affinity for interacting proteins. Additionally, intracellular signaling pathways, calcium levels, and changes in subcellular localization can influence AKAP5 function and its interactions with other molecules.
Due to its vital role in regulating various cellular processes, including neuronal function, AKAP5 has been considered as a potential therapeutic target. Developing drugs or compounds that selectively modulate AKAP5 interactions or disrupt specific protein-protein interactions involving AKAP5 could provide a means to regulate neuronal signaling and potentially treat certain neurological disorders.
Customer Reviews (4)
Write a reviewThe AKAP5 protein stands as a pinnacle of quality, demonstrating exceptional purity and functionality that elegantly fulfills my experimental requirements.
Beyond the protein's outstanding attributes, the manufacturer's commitment to delivering excellent technical support is truly remarkable.
By utilizing the AKAP5 protein, I can confidently delve into my experiments, knowing that it will provide unparalleled precision and effectiveness.
Its superior composition ensures utmost reliability and accuracy in achieving my research objectives.
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