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Recombinant Human AKAP8L 293 Cell Lysate

Cat.No. : AKAP8L-8936HCL
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  • Gene Information
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Description : Antigen standard for A kinase (PRKA) anchor protein 8-like (AKAP8L) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : AKAP8L A kinase (PRKA) anchor protein 8-like [ Homo sapiens ]
Official Symbol : AKAP8L
Synonyms : AKAP8L; A kinase (PRKA) anchor protein 8-like; A-kinase anchor protein 8-like; HAP95; NAKAP95; neighbor of A kinase anchoring protein 95; AKAP8-like protein; neighbor of AKAP95; homologous to AKAP95 protein; helicase A-binding protein 95 kDa; neighbor of A-kinase anchoring protein 95; neighbor of A-kinase-anchoring protein 95; HA95; NAKAP; DKFZp434L0650;
Gene ID : 26993
mRNA Refseq : NM_014371
Protein Refseq : NP_055186
MIM : 609475
UniProt ID : Q9ULX6
Chromosome Location : 19p13.13-p13.12
Function : DEAD/H-box RNA helicase binding; DNA binding; metal ion binding; protein binding; zinc ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (12)

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Is AKAP8L involved in cancer? 10/25/2021

The role of AKAP8L in cancer is still being actively studied. Some research suggests that AKAP8L can promote cell proliferation and survival, and its dysregulation has been observed in certain cancer types. However, more studies are needed to fully understand its involvement in cancer development and progression.

Is AKAP8L implicated in any diseases or disorders? 02/23/2021

AKAP8L dysregulation has been implicated in certain diseases and disorders. For example, alterations in AKAP8L expression or function have been observed in various types of cancer, including breast cancer, ovarian cancer, and neuroblastoma. It has also been associated with neurodevelopmental disorders such as intellectual disability.

Are there any known post-translational modifications of AKAP8L? 05/07/2018

Yes, AKAP8L undergoes post-translational modifications that can influence its activity and interactions. Phosphorylation, as mentioned earlier, is one such modification that has been identified. Other potential modifications include acetylation, methylation, and ubiquitination, although further studies are needed to confirm and characterize these modifications.

Are there any animal models or knockout studies for AKAP8L? 09/21/2017

While there may not be extensive knockout studies specifically targeting AKAP8L, some studies have used small interfering RNA (siRNA) or CRISPR-Cas9 techniques to knock down AKAP8L expression in cell lines. These studies have provided insights into the protein's functions and its involvement in various cellular processes.

Are there any known interacting proteins of AKAP8L? 03/16/2017

Yes, AKAP8L has been found to interact with several proteins involved in various cellular processes. Some known interacting proteins include protein kinase A (PKA), cyclin-dependent kinase 1 (CDK1), DNA repair proteins such as PARP1 and XRCC5, chromatin-associated proteins like histones, and transcription factors like p53.

Is AKAP8L involved in cellular signaling pathways? 03/01/2017

AKAP8L itself does not directly participate in classical cellular signaling pathways. Instead, it acts as a platform for interaction with other proteins involved in signaling pathways. For example, AKAP8L can associate with PKA and facilitate its interaction with transcription factors, thus modulating gene expression. AKAP8L's role is more closely related to gene regulation and DNA repair.

Can AKAP8L phosphorylation affect its function? 01/20/2017

Yes, AKAP8L contains multiple phosphorylation sites, and phosphorylation can regulate its interactions and function. For example, phosphorylation by CDK1 promotes the dissociation of AKAP8L from chromatin during mitosis. Additionally, phosphorylation events may modulate its interactions with other proteins, affecting gene regulation and DNA repair processes.

Are there any known genetic mutations or disorders associated with AKAP8L? 01/14/2017

Currently, there are no known genetic mutations or disorders specifically associated with AKAP8L. However, genetic variations or dysregulation of AKAP8L expression may contribute to certain diseases or conditions, and further investigations are needed to explore these associations.

Can AKAP8L be targeted for therapeutic purposes? 09/10/2016

Since AKAP8L plays a role in various cellular processes, targeting it could have therapeutic potential. However, further research is required to fully understand its functions and the implications of targeting it for specific diseases or conditions.

Does AKAP8L play a role in DNA repair? 05/06/2016

Yes, AKAP8L is involved in DNA repair processes. It interacts with DNA repair proteins, such as PARP1 and XRCC5, and contributes to the recruitment of repair factors to DNA damage sites. Studies suggest that AKAP8L may play a role in both base excision repair and non-homologous end joining pathways.

Is AKAP8L associated with any specific cellular pathways? 12/02/2015

AKAP8L is involved in multiple cellular pathways. It plays a role in cell cycle progression, gene transcription, DNA repair, mitosis, and chromatin remodeling. Its interactions with proteins and involvement in various cellular processes suggest its importance in maintaining cellular homeostasis.

Can AKAP8L interact with other AKAPs (A-kinase anchoring proteins)? 07/03/2015

While AKAP8L is a member of the AKAP family, it does not directly interact with other AKAPs. AKAPs typically interact with protein kinase A (PKA) and localize it to specific subcellular compartments. AKAP8L, on the other hand, functions as a scaffold protein in gene regulation and DNA repair processes, interacting with a different set of proteins.

Customer Reviews (4)

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Reviews
06/03/2021

    Its superior composition assures reliability, precision, and consistent outcomes, making it the ideal protein for my research endeavors.

    10/24/2017

      Their dedication to providing excellent assistance inspires confidence in their ability to tackle any challenges that may arise.

      05/23/2017

        The Akap8l protein's unrivaled attributes are poised to not only meet but surpass my experimental needs.

        01/21/2016

          The Akap8l protein exemplifies exceptional quality, characterized by its remarkable purity and functionality, perfectly aligning with the demands of my experimental requirements.

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