Recombinant Human AKR1A1

• Cat.No.: AKR1A1-485H
• Product Overview: Recombinant humanAlcohol dehydrogenase was expressed inE.coliand purified by using conventional chromatography techniques. MW =36.5 kDa (325aa).

Specification

• Cat. No.: AKR1A1-485H
• Description: Alcohol dehydrogenase [NADP+] (AKR1A1) is a member of the aldo/keto reductase superfamily and catalyzes the NADPH-dependent reduction of a variety of aromatic and aliphatic aldehydes to their corresponding alcohols. This protein is closely related (65% identity) to aldose reductase, an enzyme involved in the pathogenesis of some diabetic and galactosemic complications. It plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN).
• Source: E.coli.
• Amino Acid Sequence: MAASCVLLHT GQKMPLIGLG TWKSEPGQVK AAVKYALSVG YRHIDCAAIY GNEPEIGEAL KEDVGPGKAV PREELFVTSK LWNTKHHPED VEPALRKTLA DLQLEYLDLY LMHWPYAFER GDNPFPKNAD GTICYDSTHY KETWKALEAL VAKGLVQALG LSNFNSRQID DILSVASVRP AVLQVECHPY LAQNELIAHC QARGLEVTAY SPLGSSDRAW RDPDEPVLLE EPVVLALAEKYGRSPAQILL RWQVQRKVIC IPKSITPSRI LQNIKVFDFT FSPEEMKQLN ALNKNWRYIV PMLTVDGKRV PRDAGHPLYP FNDPY
• Form: Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 50mM NaCl, 10% glycerol.
• Purity: > 90% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: AKR1A1 aldo-keto reductase family 1, member A1 (aldehyde reductase) [ Homo sapiens ]
• Synonyms: AKR1A1; aldo-keto reductase family 1, member A1 (aldehyde reductase); ALR; ARM; DD3; ALDR1; MGC1380; MGC12529; OTTHUMP00000009240; aldehyde reductase; alcohol dehydrogenase; dihydrodiol dehydrogenase 3; EC 1.1.1.2; Alcohol dehydrogenase [NADP+]; Aldo-keto reductase family 1 member A1; OTTHUMP00000009241; aldo-keto reductase family 1, member A1
• Gene ID: 10327
• mRNA Refseq: NM_006066
• Protein Refseq: NP_006057
• MIM: 103830
• UniProt ID: P14550
• Chromosome Location: 1p33-p32
• Pathway: Glycerolipid metabolismC; Glycolysis / Gluconeogenesis; Metabolic pathways
• Function: L-glucuronate reductase activity; alcohol dehydrogenase (NADP+) activity; aldehyde reductase activity; electron carrier activity; oxidoreductase activity; protein binding