Recombinant Human Alanyl-tRNA Synthetase, His-tagged

• Cat.No.: AARS-51H
• Product Overview: Recombinant HumanAlanyl-tRNA synthetase produced inSf9is a glycosylated, polypeptide chain having a molecular mass of 107,598 Dalton. PL-12 is expressed with a -6x His tag and purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Description: Alanyl-tRNA synthetase is a member of the aminoacyl-tRNA synthetase family, key enzymes of protein biosynthesis which charge tRNA molecules with the respective amino acids. This 108 kDa protein is an autoantigen recognized by PL-12 antibodies which occur in a subset of patients with polymyositis and dermatomyositis. Preliminary data suggest that epitope spreading occurs in the autoimmune PL-12 response such that even antibodies to an isolated alanyl-tRNA molecule can develop.
• Physical Appearance: Sterile Filtered clear solution.
• Purity: Greater than 90% as determined by SDS-PAGE.
• Formulation: PL-12 is supplied at a of 0.9 mg/ml in16 mM HEPES buffer pH-8, 250 mM sodium chloride, and 20% glycerol.
• Immunological Functions: 1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels immuno-dot test).
• Applications: Western-Blot with monoclonal anti-hexa-His-tag antibody & Polymyositis sera.
• Coating Concentration: 0.3-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
• Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
• Publications:
  Evaluation of paraneoplastic antigens reveals TRIM21 autoantibodies as biomarker for early detection of ovarian cancer in combination with autoantibodies to NY-ESO-1 and TP53 (2020)

Gene Information

• Gene Name: AARS alanyl-tRNA synthetase [ Homo sapiens ]
• Synonyms: AARS; alanyl-tRNA synthetase; alanine tRNA ligase 1, cytoplasmic; Alanyl-tRNA synthetase, cytoplasmic; Renal carcinoma antigen NY-REN-42; Alanine--tRNA ligase; AlaRS; EC 6.1.1.7
• Gene ID: 16
• mRNA Refseq: NM_001605
• Protein Refseq: NP_001596
• MIM: 601065
• UniProt ID: P49588
• Chromosome Location: 16q22
• Pathway: Aminoacyl-tRNA biosynthesis; Gene Expression
• Function: ATP binding; alanine-tRNA ligase activity; ligase activity; nucleic acid binding; tRNA binding

Citation

Evaluation of paraneoplastic antigens reveals TRIM21 autoantibodies as biomarker for early detection of ovarian cancer in combination with autoantibodies to NY-ESO-1 and TP53

Journal: Cancer biomarkers : section A of Disease markers    PubMed ID: 32083570    Data: 2021/4/27

Authors: Laura C. Hurley, Nancy K. Levin, Michael A. Tainsky

Article Snippet:The recombinant proteins in this study are full-length, with the exception of the His-tagged recombinant PAX8 protein that consists of the amino acids 1–287 (Sino Biological, Wayne, PA, USA).The recombinant proteins in this study are full-length, with the exception of the His-tagged recombinant PAX8 protein that consists of the amino acids 1–287 (Sino Biological, Wayne, PA, USA).. The full-length AARS His-tagged recombinant protein (AARS-51H) was purchased, and was produced in Sf9 insect cells (Creative BioMart, Shirley, NY, USA).