Active Recombinant Human ALCAM protein, Fc-tagged

• Cat.No.: ALCAM-2864H
• Product Overview: Active Recombinant Human ALCAM protein(Q13740-1)(Trp28-Ala526) is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 28-526 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human CD6, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human ALCAM, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 82.7 kDa. Due to glycosylation, the protein migrates to 90-115 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per ug by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: ALCAM activated leukocyte cell adhesion molecule [ Homo sapiens ]
• Official Symbol: ALCAM
• Synonyms: ALCAM; activated leukocyte cell adhesion molecule; activated leucocyte cell adhesion molecule; CD166 antigen; CD166; MEMD; FLJ38514; MGC71733
• Gene ID: 214
• mRNA Refseq: NM_001243280
• Protein Refseq: NP_001230209
• MIM: 601662
• UniProt ID: Q13740