Recombinant Human AMACR, His-tagged

• Cat.No.: AMACR-107H
• Product Overview: Recombinant Human AMACR, transcript variant 2, fused with N-terminal HIS, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene.
• Molecular Mass: 22 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method
• Storage Buffer: 25mM Tris, pH8.0, 150mM NaCl, 10% glycerol, 1 % Sarkosyl.

Gene Information

• Gene Name: AMACR alpha-methylacyl-CoA racemase [ Homo sapiens (human) ]
• Official Symbol: AMACR
• Synonyms: AMACR; RM; RACE; CBAS4; AMACRD; alpha-methylacyl-CoA racemase; alpha-methylacyl-CoA racemase; 2-methylacyl-CoA racemase; NP_001161067.1; EC 5.1.99.4; NP_055139.4; NP_976316.1
• Gene ID: 23600
• mRNA Refseq: NM_203382
• Protein Refseq: NP_976316
• MIM: 604489
• UniProt ID: Q9UHK6
• Chromosome Location: 5p13
• Pathway: Beta-oxidation of pristanoyl-CoA; Bile acid biosynthesis, cholesterol => cholate/chenodeoxycholate; Peroxisomal lipid metabolism
• Function: alpha-methylacyl-CoA racemase activity; receptor binding