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Recombinant Human ANAPC4 protein, GST-tagged

Cat.No. : ANAPC4-545H
Product Overview : Human ANAPC4 partial ORF ( AAH59383, 651 a.a. - 750 a.a.) recombinant protein with GST-tag at N-terminal.
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  • Gene Information
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Description : A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The exact function of this gene product is not known. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
Source : Wheat Germ
Species : Human
Tag : GST
Molecular Mass : 36.63 kDa
AA Sequence : VVLKDTVGREGRDRLLVQLPLSLVY NSEDSAEYQFTGTYSTRLDEQCSAI PTRTMHFEKHWRLLESMKAQYVAGN GFRKVSCVLSSNLRHVRVFEMDIDD
Applications : Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
Notes : Best use within three months from the date of receipt of this protein.
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Gene Name : ANAPC4 anaphase promoting complex subunit 4 [ Homo sapiens ]
Official Symbol : ANAPC4
Synonyms : ANAPC4; anaphase promoting complex subunit 4; anaphase-promoting complex subunit 4; APC4; cyclosome subunit 4;
Gene ID : 29945
mRNA Refseq : NM_013367
Protein Refseq : NP_037499
MIM : 606947
UniProt ID : Q9UJX5

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (21)

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Are there any known diseases or conditions associated with ANAPC4 mutations? 10/25/2022

Currently, there are no specific diseases or conditions directly associated with ANAPC4 mutations. However, mutations in other subunits of the APC/C complex have been linked to developmental disorders, intellectual disabilities, and various types of cancer.

Is ANAPC4 involved in any other cellular processes besides cell cycle regulation? 05/15/2022

Yes, ANAPC4 has been implicated in other cellular processes beyond cell cycle regulation. It has been found to have roles in DNA repair, chromatin remodeling, transcriptional regulation, and centrosome duplication.

Does ANAPC4 play a role in centrosome duplication? 04/19/2022

Yes, ANAPC4 has been implicated in the regulation of centrosome duplication, which is essential for proper mitotic spindle formation. It interacts with proteins involved in centrosome duplication, such as Plk1, and its dysregulation can lead to abnormal centrosome duplication and mitotic defects.

What experimental techniques are used to study ANAPC4 protein? 08/30/2021

Various experimental techniques are used to study ANAPC4 protein, including biochemical analyses, immunoprecipitation, immunofluorescence, genetic approaches (gene knockout or knockdown), and cell cycle analysis. Additionally, structural studies, such as X-ray crystallography or cryo-electron microscopy, can provide insights into the protein's structure-function relationship.

How is ANAPC4 involved in cell cycle regulation? 02/22/2021

ANAPC4 is a crucial component of the APC/C complex, which plays a central role in cell cycle regulation. During mitosis, the APC/C complex is activated and targets specific proteins for degradation through ubiquitin-mediated proteolysis. ANAPC4, along with other subunits of the APC/C complex, helps recognize and bind to specific target proteins, marking them for degradation and allowing proper progression through the cell cycle.

Are there any known inhibitors or modulators of ANAPC4 activity? 12/25/2020

Currently, there are no specific inhibitors or modulators specifically targeting ANAPC4. However, inhibitors of the APC/C complex, such as proTAME, have been developed and shown promising effects in preclinical studies. These inhibitors may indirectly affect ANAPC4 activity within the APC/C complex.

Is ANAPC4 conserved across different species? 10/21/2020

Yes, ANAPC4 is highly conserved across different species, from yeast to humans. This conservation suggests its importance in fundamental cellular processes and highlights its essential role in cell cycle regulation and other cellular functions.

Are there any ongoing clinical trials targeting ANAPC4 or the APC/C complex? 10/13/2019

There are currently no known clinical trials specifically targeting ANAPC4 or the APC/C complex. However, targeting the APC/C complex as a whole has been explored in preclinical studies and has shown potential for therapeutic interventions in cancer treatment.

Can ANAPC4 mutations contribute to cancer development? 08/02/2019

Yes, mutations in ANAPC4 and other subunits of the APC/C complex have been found in various types of cancer. Dysregulation of the APC/C complex can lead to abnormal cell cycle progression, genomic instability, and unchecked cell growth, which are all hallmarks of cancer development.

How is ANAPC4 involved in DNA repair processes? 06/16/2018

ANAPC4, as part of the APC/C complex, has been found to interact with proteins involved in DNA repair, such as BRCA2 and RAD51. These interactions suggest a role for ANAPC4 in regulating DNA repair processes, particularly homologous recombination.

Are there any known genetic disorders associated with mutations in ANAPC4? 05/23/2018

Currently, there is no specific genetic disorder that has been directly linked to ANAPC4 mutations. However, mutations in other subunits of the APC/C complex have been associated with developmental disorders and intellectual disabilities.

How is ANAPC4 protein regulated? 02/03/2018

The activity of ANAPC4 is tightly regulated through multiple mechanisms. Its expression is regulated during the cell cycle, and its association with other subunits of the APC/C complex is crucial for its activity. Additionally, post-translational modifications, such as phosphorylation, can modulate ANAPC4 function.

Are there any diseases or disorders associated with ANAPC4 dysfunction? 01/14/2018

Mutations or dysregulation of APC/C complex subunits, including ANAPC4, have been implicated in various human diseases and disorders, including certain types of cancer, neurodegenerative diseases, and developmental disorders.

Does ANAPC4 have any post-translational modifications? 07/20/2017

Yes, ANAPC4 can undergo post-translational modifications, including phosphorylation. Phosphorylation of ANAPC4 can regulate its activity and interactions within the APC/C complex.

Can ANAPC4 interact with other cellular pathways or signaling networks? 07/15/2017

Yes, ANAPC4 can interact with other cellular pathways and signaling networks. It has been implicated in DNA repair processes, such as homologous recombination, and can also affect transcriptional regulation through interactions with transcription factors or co-regulators. Further studies are needed to fully understand these interactions.

Can ANAPC4 be used as a diagnostic marker for certain diseases? 01/27/2017

Currently, ANAPC4 is not widely used as a diagnostic marker for specific diseases. However, further research may uncover its potential as a diagnostic marker or therapeutic target in certain conditions.

Can ANAPC4 be targeted for therapeutic interventions? 07/03/2016

Targeting ANAPC4 directly as a therapeutic intervention is challenging due to its essential role in the APC/C complex and its involvement in multiple cellular processes. However, targeting the APC/C complex as a whole has been explored as a potential therapeutic strategy, particularly in the context of cancer treatment.

Is ANAPC4 protein essential for cell viability? 06/15/2016

Yes, ANAPC4 is essential for cell viability and proper cell cycle progression. The loss or dysfunction of ANAPC4 can lead to cell cycle defects and impaired chromosome segregation.

Can ANAPC4 affect transcriptional regulation? 05/07/2016

Yes, ANAPC4 has been shown to interact with transcription factors and co-regulators, suggesting a role in transcriptional regulation. It may modulate gene expression by influencing the stability and activity of transcription factors or by regulating the turnover of specific proteins involved in transcription.

Are there any inhibitors or drugs targeting ANAPC4? 05/04/2016

Currently, there are no specific inhibitors or drugs targeting ANAPC4. However, targeting the APC/C complex as a whole has been explored as a potential therapeutic strategy in certain cancers.

Does ANAPC4 have any other known functions outside of the cell cycle regulation? 03/06/2016

While the primary known function of ANAPC4 is its role in the cell cycle regulation, emerging evidence suggests potential roles in other cellular processes, such as DNA repair and transcriptional regulation.

Customer Reviews (5)

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Reviews
03/08/2021

    The manufacturer's team of experts possesses a deep understanding of the ANAPC4 protein and its associated protocols.

    07/28/2020

      One of the key advantages of using ANAPC4 protein in trials is its high quality.

      09/16/2019

        This high-quality ANAPC4 protein provides researchers with confidence in obtaining accurate and reliable data during their investigations

        01/24/2019

          The manufacturer ensures that the protein is produced with utmost purity and integrity, minimizing the risk of experimental artifacts or compromised results.

          01/05/2019

            In conclusion, using ANAPC4 protein in trials offers distinct advantages for researchers studying cell cycle regulation.

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